Biology

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MOLECULAR DETECTION OF CITRUS VIROIDS IN WEST BANK
(جامعة النجاح الوطنية, 2022-02-11) Abualrob, Aswar
Palestine as many of developing countries; its economy depends on many factors including agriculture sector. The citrus cultivated areas for the year 2012/2013, in the West Bank reached approximately 9.831 hectares, which constituted to 9.2% of the total area of fruit trees with annual production of approximately 4% of total production. Citrus are natural hosts to several pathogens like: bacteria, fungi, viruses, and virus-like (viroid). Many of which have an economic ipmpact on the crop A minimum of five viroid species of Pospiviroidae family that are found in citrus trees. The purpose of this study was detection of five citrus viroids (CVdIII , CVdIV , CEVd, HSVd and CBLVd) in West bank by molecular means. Field surveys were conducted during august 2020; where a total of 42 samples were collected from citrus collection plot in NRAC –Jenin. Samples screening for presence of any viroids species were carried out by two-steps RT-PCR. Five viroid were detected by RT-PCR for the first time in West bank. The incidence of Citrus viroid III, Citrus exocortis viroid (CEVd), and Citrus viroid IV were found 35.7%, 23.8%, and 16.6% respectively. Meanwhile Hop stunt viroid and Citrus bent leaf viroid were found in 9.5% and 7%. Portion of CEVd, CVdIII and CVdIV were sequenced to be later deposited in gene bank as Palestinian isolate.