GENERATION OF ENGINEERED DERMAL TISSUES ENRICHED WITH CHITOSAN-MULTIWALL CARBON NANOTUBE COMPLEX FOR UTILIZATION IN WOUND HEALING
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Date
2023-05-29
Authors
Amal Jafar Alqato
Journal Title
Journal ISSN
Volume Title
Publisher
An - Najah National University
Abstract
Introduction and background: The process of wound healing is complicated and involves four precise stages: hemostasis, inflammation, proliferation, and remodeling. When interrupted, this process can result in chronic wounds, constituting significant health and economic burden. Engineered skin tissues (EST) with different constituents have been proposed as a potential treatment.
Aim of the project: To generate engineered dermis tissues (EDTs) as a substitute for the dermis layer to enhance wound healing.
Material and Methods: The scaffolds of the generated EDTs were based on collagen, which is similar to the natural dermis, and it was enriched with chitosan, a natural biocompatible and biodegradable polymer that possesses wound-healing properties, and different concentrations of multiwall carbon nanotubes (MWCNTs) that can enhance the mechanical properties of the EDTs. The effect of incorporating angiotensin-II (Ang II) in the tissues on angiogenesis was also investigated. All tissues were populated by 3T3 cells. The EDTs were transplanted in a mouse wound model, and the wound sites were analyzed macroscopically and histologically by masson-trichrome stain after 14 days of transplantation to evaluate the quality of wound healing.
Results: Overall, our study found that transplanted tissue had no negative impact on animal health. It reduced contraction and facilitated epithelization, but did not affect the percentage of wound closure. EDTs transplantation did not affect the thickness of the new epidermis, but it increased the thickness of the dermis. The incorporation of Ang II in the matrix of the EDT did not affect the degree of angiogenesis.
Conclusions and Recommendations: The transplanted tissue enhanced the quality of wound healing by promoting epithelialization and reducing contraction. This finding is significant for the development of potential treatments for slow-healing or high-risk scarring wounds.