Green Synthesis of Carbon Nano-dots from Herbs and Plants for Biomedical Applications
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Date
2023-07-06
Authors
Amal Barham
Hala Badawi
Zeina Barham
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Abstract
Abstract
Carbon nanodots, a family of carbon-based nanomaterials, have been synthesized through different methods from various resources, affecting the properties of the resulting product and their application. Carbon nanodots (CNDs) were synthesized with a green and simple hydrothermal method to prepare CNDs from sage leaves at 200˚C for 6 hours. The resulted CNDs are well dispersed in water due to their negative surface charge (ζ-potential = -11mV). The CNDs synthesized from sage have an average particle size of 3.6 nm. The synthesized CNDs showed excellent anticancer activity toward liver cancer (Hep3B) cell lines, the cells treated with CNDs at 50-250 µg/ml concentrations and resulting that the cell activity decreased with increasing the concentration from 92% at 50 µg/ml to reach 23% at 250 µg/ml. In addition, the cytotoxicity of the CNDs was analyzed in a liver normal cell (LX2) resulting that the CNDs at a concentration of 50 µg/ml were still active with 98.5%, also 86.7% of the LX2 activity was maintained at a high concentration (250 µg/ml CNDs). Moreover, the CNDs have hindered the formation of the 3D spheroidal from Hep3B. Herein, it would be concluded that the synthesized CNDs from sage are higher selective to liver cancer cells than normal ones. The CNDs' cancer-killing ability would be referred to the production of reactive oxygen species (ROS).