Pharmacy & Pharm.D
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- ItemAlkanna use in Cosmetics and Medicine(2017-05-25) Basil Fuqaha; Ahmad Nawahda; Maha Assadi; Dr.Nidal Jaradat
- ItemAnalytical Method Development and Validation for Quantification of Ceftiofur Hydrochloride in Veterinary Oily Suspension Using HPLC Coupled with UV Detector(2021-05-31) Naser Shraim; Marah Ali Husain; Sae’d Mazen IDK; Amani Ismail
- ItemAnalytical Method Development and Validation of High-Performance Liquid Chromatography for Simultaneous Determination of Ibuprofen and Baclofen(2017-04-25) Aman Khasati; Aseel Ben Ali; Noor Souqi; Dr. Naser Shraim
- ItemAnalytical Method Development and Validation of High-Performance Liquid Chromatography for Simultaneous Determination of Oxytetracycline Hydrochloride and Flunixin in Veterinary Injectable Solution(2021-08-26) Rand Abdullah; Aseel Mansour; Mais Mansour; Naser ShraimA linear, precise, specific and accurate high performance liquid chromatographic validation method was developed for determination of Oxytetracycline and Flunixine in an injectable pharmaceutical dosage form (Fenox®). The purpose of high performance liquid chromatography (HPLC) analysis of any drug is to confirm the identity of a drug and provide quantitative results and also to monitor the progress of the therapy of a disease. The aim of the study is to develop and validate a liquid chromatographic method to quantify and determine two active ingredients simultaneously in one dosage form. The proposed method is rapid, cost-effective and can be used as a quality-control tool for routine quantitative analysis of these two active ingredients in the injectable dosage form. The HPLC analysis was performed on the phenomixc18, 15cm column, which was of a diameter of 4.5 mm and a particle size of 5 µm. Different mobile phases were then tested by using different mixture of ACN and buffer solution to obtain a suitable retention time and a pH 8.5. The UV detection was performed at the wavelength (λmax) range of 200-500 nanometers and the retention time of ocytetracycline 1.343 and flunixine 4.573 min. Analytical method validation was done in accordance to ICH guidelines. The linearity of the calibration curve was then established in the concentration range of 0.16-0.64 mg\ml of Oxytetracycline and 0.032-0.128 mg/ml of Flunixine. The mean recovery of 101.4 % -100.2%. The values of LOD Oxytetracycline 0.011949 flunixine 0.005281 and LOQ oxytetracycline 0.036209 flunixine 0.016003. According to the accuracy outcome results the RSD value for all Oxytetracycline samples is 0.283841 and for Flunixine samples is 0.304195, which represents good accuracy of the used method. As well the developed method showed excellent linearity, accuracy, precision, specificity, robustness, LOD and LOQ results within the acceptance criteria.
- ItemAnalytical Method Development for Sodium Valporate through Chemical Derivatization(2018-05-25) Nihaya Wasif Odeh; Ghadeer Naser Younis; Oyoun Fadel Zeidan; Dr. Murad AbualhasanBackground: Sodium valporate has anticonvulsant activity and is structurally different than conventional antiepileptic drug.Valporic acid lack conjugation in its structure and this makes it only detected a very short wavelength with low detection sensitivity . The objective of the study is to derivatized sodium valporate and introduce an conjugation to its structure to increase its absorbtion under UV detection range. The developed method can be adapted in routine analysis of valporic acid in pharmaceutical dosage as well as biological system.. Methodology: Sodium valporate was derivatized by adding a chromophore to its structure through introducing a methyl benzoyl or a phenyl group. Two reagents were used namely trichlorophenol and 2-hyddroxyacetophenone to introduce phenyl and benzoyl group to valporic acid respectively. The reaction used was estrification reaction using coupling agents. An analytical method was then developed and validated using reverse phase HPLC. The method was validated for parameters like linearity, range, accuracy precision and robustness. Results: The developed method was easy and feasible and can be applied to both routine analysis and bioanalysis. The method was very sensitive and could quantify valporic acid at a very low quantity. The developed method was found to be linear, accurate precise and robust. Conclusion: The proposed chemical derivatization and the developed analytical method is novel. The developed analytical procedure is the first of its kind, it is easy and feasible and can used to quantify and detect sodium valporate at very low concentration compared to other available methods available in the literature
- ItemAnti-obesity Activity for 14 Wild Plants from Palestine(2016-04-20) Eyass Zuhair Zaghal; Maram Mahmoud Zaqzouq; Hadeel Jawad Aljammal; Dr. Nidal JaradatAnti-obesity activity was measured by using porcine pancreatic lipase inhibitory test which was established by using spectrophotometer method and used orlistat anti-obesity drug as appositive control reference. Results: The porcine pancreatic lipase inhibitory effect for organic and aqueous extracts of the studied 14 plants showed antilipase activity. Conclusions: The results showed that the studied plants have antiobesity activity, providing evidence for its folkloric use as functional food and medicine.
- ItemAntibacterial and Anticancer properties of Palestinian Asparagus(2018-05-26) Zaina Samer Barah; Zeina Wasfi Jarawan; Shahd Nedal Mahmoud; Dr. Nidal Jaradat
- ItemAnticancer activity of Holy Bramble plant(2019-05-25) Jalal Abu Asab; Khalil Obeid; Mohammad Sharabati; Dr. Nidal Jaradat
- ItemAnticancer Activity of Novel Amide Methoxyphenyl Derivatives(2019-05-25) Osama Hamoudi Eman Nawasra Abdullah abdelrazaq; Eman Nawasra; Abdullah abdelrazaq; Dr. Mohammad HawwashWorldwide, cancer is being the most killer disease, (1,1-2) 25% of the deaths in the 3rd world country are due to cancer. It has killed 9.6 million humans in 2018, out of 5 men, one is developing cancer during his life and for women the ratio is 1 out of 6. Many environmental factor as diet, obesity, alcohol consumption, physical inactivity, radiation, sunlight, viral infection and other genetic factors scratch together leading to this fateful disease. Over the years scientists invented a lot of treatments for cancer, each type of cancer is tried to be cured by its own therapy as chemotherapy, hormonal, radiation and surgery. Four compounds were synthesized as isoxazole-carboxamide. Characterized by HRMS, CNMR and HNMR. Compounds OAE4 showed potent anticancer against Hep3B cancer cell line. By further investigating their molecular effectors, it was revealed that compound OAE4 caused cell cycle arrest at the G2/M phase and it was close to Doxorubicin value as reference. In future studies, we can synthesize more analogues of carboxamide-Isoxazole derivatives as promising anticancer agents and further in-vivo investigations required to approve these effects and to design suitable pharmaceutical dosage forms from these compounds.
- ItemAnticancer, Antimicrobial, Antioxidant Properties and Preliminary Phytochemical Screening Of Dittrichia Viscosa (L.) Greuter, Taraxacum Cyprium H.Lindb. and Eruca Vesicaria (L.) Cav.(2016-04-30) Aya Tamimi; Abdullah Almasri; Waleed Abu Najeeb; Dr. Johnny AmerBackground Background cancer  has become a serious, global concern, and so the discovery of novel antioxidant and antimicrobial from herbal sources may provide anticancer and valuable solutions for this problem. Actually, replacement of harmful chemical antioxidants with natural ones may prevent various diseases. The present investigation describes for the first time the antioxidant , phytochemical screening , Anticancer  and antimicrobial activities of aqueous and organic extracts to the roots of  Dittrichia Viscosa, Taraxacum Cyprium and Eruca Vesicaria . Methods: Free radical scavenging property was analyzed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, while antimicrobial activity was tested against the selected strains from American Type Culture Collection (ATCC) and clinical isolates such as Shigella sonnie, Staphylococcus aureus, Enterococcussfeacium, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Epidermophyton floccosum and Methicillin Resistant Staphylococcus aureus (MRSA), and using minimum inhibitory concentration (MIC) assay. Results: A mixture of phytochemical compounds was found in all of the studied extracts which also showed remarkable potentials of antioxidant , and antimicrobial activities. The current study provides initial data that justify the use and importance of these plants in Palestinian traditional medicine. Conclusion: This study provides evidence that the aqueous and organic extracts from the roots of Dittrichia Viscosa, Taraxacum Cyprium , and Eruca Vesicariaexhibited interesting antioxidant activities when compared to Trolox ,
- ItemAntimicrobial and Anti-cancer Activity of Purslane: An Experimental Test of its Oil Mechanism of Action(2021-05-07) Ihdaa` Ibraheem; Batool Salman; Siwar Nashif; Ahmad Eid
- ItemAntimicrobial and antioxidant activities of B. lancifolium(2016-05-30) Amanda Aker; Dalal Qasem; Saja Hejazi; Dr.Nidal JaradatMethods: Antioxidant activities of four plant fractions were evaluated by DPPH scavenging assay, while the antimicrobial activity was assessed by broth microdilution method. The antimicrobial activity of four plant fractions was examined American Type Culture Collection reference strains. Results:The results showed antibacterial and antioxidant activity for the studied plant extracts. Further investigations (isolation, identification, and clinical assessment) of the active compound(s) are needed for the possible formulation of new therapeutic alternatives.
- ItemAntimicrobial and antioxidant activities of B. lancifolium(2016-05-20) Amanda Aker; Dalal Qasem; Saja Hejazi; Dr.Nidal JaradatMethods: Antioxidant activities of four plant fractions were evaluated by DPPH scavenging assay, while the antimicrobial activity was assessed by broth microdilution method. The antimicrobial activity of four plant fractions was examined American Type Culture Collection reference strains. Results:The results showed antibacterial and antioxidant activity for the studied plant extracts. Further investigations (isolation, identification, and clinical assessment) of the active compound(s) are needed for the possible formulation of new therapeutic alternatives.
- ItemAntioxidant , Amylase , Lipase , SPF, Antimicrobial and Cytotoxic Test of Chia seed oil(2021-05-19) Hadeel Hamaydeh; Aya Khasati; Saba` AbuElhaja; Ahmad Eid
- ItemAntioxidant, Anti Lipase, Anti Amylase Activity Evaluation of Basil (Ocimum Basilicum) Seeds Oil in Palestine(2021-06-28) Mohammad Shakhsheer; Noor Nawahdah; Ali Hanbali; Ahmad Eid
- ItemArsenic in Rice(2013-05-25) Saja Ibrahim El-hour; Sadeya Abu Radi; Hilda Salah Titi; Dr. Hamzeh Al ZabadiArsenic (As) is considered one of the most important toxic elements in the environment because of its potential risk to human and to ecosystem. Arsenic is a relatively inert, odorless, white or colorless Tasteless powders that do not evaporate. A part from drinking water, rice consumption may be the most important pathway of human arsenic uptake. Arsenic could be found in two forms, either inorganic or organic substances. An inorganic arsenic compound is more toxic which used in pesticides. The World Health Organization (WHO) and the U.S. Environmental Protection Agency have recommended a threshold of 10 μg/L for inorganic arsenic concentration in drinking water. The International Agency for Research on Cancer (IARC) classifies arsenic as (Group 1) human carcinogen. Brown rice contains arsenic more than white rice. Countries produce rice do not affect the production country only, but also other rice imported countries could be affected around the world who import rice mainly from India, China, Thailand and California
- ItemAssessment of age-related Changes in Pediatric Gastrointestinal Solubility of Azithromycin in Biorelevant Media(2018-05-30) Haya Taffal; Amani Omar; Bisan Dwikat
- ItemAssessment of age-related Changes in Pediatric Gastrointestinal Solubility of Azithromycin in Biorelevant Media(2018-05-25) Haya Taffal; Amani Omar; Bisan Dwikat; Dr.Ramzi ShawahnaBackground: Solubility of a drug in the gastrointestinal (GI) tract is an important determinant of its oral bioavailability as only dissolved drugs are absorbed from the GI tract. Adults and children have different physiological and anatomical characteristics, which consequently may lead to differences in the pharmacokinetics of drugs and consequently lead to different exposure-response. Azithromycin is widely used as an antibiotic for the treatment of inflammation. Solubility of azithromycin in pediatric biorelevant media was not assessed before. Objective:cThe aim of the study was to assess the solubility of azithromycin in biorelevant pediatric media and compare it with the relevant adult compounds. Methods: Solubility of azithromycin was assessed in 16 biorelevant media reflective of the gastric and proximal small intestinal environments in both fasted and fed states. Solubility assessment was conducted in a shaking water bath with a temperature set to 37 °C and 200 strokes/min. Solubility assessments were determined following a 72 h dwell period. Saturated media samples were then filtered through 0.45 μm cellulose filters and diluted with fresh media before analysis. Assessment of solubility in the fed-state media was conducted using equilibrium dialysis using dialysis membrane (MWCO 12,000–14,000 Da). For solubility assessment, dialysis membranes were removed and their contents were extracted, vortexed, centrifuged at 10,000 rpm and 37 °C for 10 min, and then filtered through 0.45 μm cellulose filters. Quantification of azithromycin was done using HPLC. Results: In the simulated fasted-gastric media, solubility of azithromycin significantly increased in biorelevant media representative of both neonates and infants. In fasted-state intestinal media, solubility was increased in media representative of pediatric media when the bile salts were assumed 150% of those of adults. Interestingly, solubility ratios were within the 80%-125% criteria which indicated that the differences in solubility of azithromycin in pediatric media compared to adult media were within the acceptable range. Conclusions: Although the solubility of azithromycin was different in pediatric biorelevant media compared to those in adults, interestingly, it did not fell outside an 80–125% range from adult values in pediatric media. Findings of this study suggest slight age-related changes in solubility of azithromycin in relation to the GI fluid composition. More investigations are needed to define a future pediatric biopharmaceutical classification system as pediatric biopharmaceutics are not well understood.
- ItemAssessment of age-related Changes in Pediatric Gastrointestinal Solubility of Azithromycin in Biorelevant Media(2018-05-25) Haya Taffal; Amani Omar; Bisan Dwikat; Dr.Ramzi ShawahnaBackground: Solubility of a drug in the gastrointestinal (GI) tract is an important determinant of its oral bioavailability as only dissolved drugs are absorbed from the GI tract. Adults and children have different physiological and anatomical characteristics, which consequently may lead to differences in the pharmacokinetics of drugs and consequently lead to different exposure-response. Azithromycin is widely used as an antibiotic for the treatment of inflammation. Solubility of azithromycin in pediatric biorelevant media was not assessed before. Objective:cThe aim of the study was to assess the solubility of azithromycin in biorelevant pediatric media and compare it with the relevant adult compounds. Methods: Solubility of azithromycin was assessed in 16 biorelevant media reflective of the gastric and proximal small intestinal environments in both fasted and fed states. Solubility assessment was conducted in a shaking water bath with a temperature set to 37 °C and 200 strokes/min. Solubility assessments were determined following a 72 h dwell period. Saturated media samples were then filtered through 0.45 μm cellulose filters and diluted with fresh media before analysis. Assessment of solubility in the fed-state media was conducted using equilibrium dialysis using dialysis membrane (MWCO 12,000–14,000 Da). For solubility assessment, dialysis membranes were removed and their contents were extracted, vortexed, centrifuged at 10,000 rpm and 37 °C for 10 min, and then filtered through 0.45 μm cellulose filters. Quantification of azithromycin was done using HPLC. Results: In the simulated fasted-gastric media, solubility of azithromycin significantly increased in biorelevant media representative of both neonates and infants. In fasted-state intestinal media, solubility was increased in media representative of pediatric media when the bile salts were assumed 150% of those of adults. Interestingly, solubility ratios were within the 80%-125% criteria which indicated that the differences in solubility of azithromycin in pediatric media compared to adult media were within the acceptable range. Conclusions: Although the solubility of azithromycin was different in pediatric biorelevant media compared to those in adults, interestingly, it did not fell outside an 80–125% range from adult values in pediatric media. Findings of this study suggest slight age-related changes in solubility of azithromycin in relation to the GI fluid composition. More investigations are needed to define a future pediatric biopharmaceutical classification system as pediatric biopharmaceutics are not well understood.
- ItemAssessment of age-related Changes in Pediatric Gastrointestinal Solubility of Cefdinir in Biorelevant Media(2017-04-25) Amr Abu-Shamaa; Ola Abu Samen; Batool Odeh; Dr.Ramzi Shawahna