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Browsing Science by Subject "ARMS-PCR"

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    Association between Genetic Thrombophilia Mutations and Homocysteine Level on In Vitro Fertilization Outcomes Effects on Positive Pregnancy Results
    (An-Najah National University, 2019-01-27) Zaid, Walaa Yasir Mahmoud
    Fertility is the normal capacity to produce offspring from two male and female cells. However, the lake of the ability to reproduce takes a place in the fertility problems, which needs an assisted reproductive technique called in vitro fertilization. That involves the injection of the male sperm in the female oocyte in embryological laboratory after treatment, to increase the chance of pregnancy. Many factors arises the need to these techniques, whether it was male causes; such as the number, motility, shape of sperm cells or a female causes; as low of fertility because of, endometriosis, ovulation disorders, mutated genes which leads to IVF failure. Thrombophilia term means the accumulation of abnormal blood clots in the blood vessels, this clots may pass through the blood stream and cause (venous thromboembolism), or may reach the lungs causing (pulmonary embolism) according to inherited or acquired causes. Homocysteine is an amino acid produced by the body, it is safe if founded in the normal range, but the elevated level meaning a many problems in the body included the narrowing of heart vessels. In this study blood samples collected from 20 women failed in IVF treatment and tested for the presence of the four mutations, then DNA extracted and certain regions of the genes were amplified using ARMS-PCR technique. The results shows that the patients with these mutations (FVL, FII, MTHFR 677, and MTHFR 1298), have also an increase in the homocysteine level because of the decrease in the enzyme, which breaks down the Hcy so that will arise the pregnancy complications. Such patients need a folate and many B vitamins to pass their genetic problems. The aim of this study is to develop a rapid and cost effective method to screen inherited thrombophilia mutations in Factor V Leiden G1691A/R506Q (FVL), prothrombin G20210A (FII) and methylenetetrahydrofolate reductase (MTHFR) C677T/ C1298A. to check the prevalence screening of genetic thrombophilia mutations among a selected samples of Palestinian women’s in IVF cycle. Then, to correlate the level of homocysteine in patients with or without the inherited thrombophilia mutations and the effect on IVF success (positive pregnancy after treatment).