ANTIMICROBIAL EFFECT OF PALESTINIAN HONEY FROM DIFFERENT BOTANICAL ORIGIN AGAINST HUMAN PATHOGENIC BACTERIAL AND FUNGAL
| dc.contributor.author | Haneen Mohammed Kamel Qalalweh | |
| dc.date.accessioned | 2024-06-09T19:48:05Z | |
| dc.date.available | 2024-06-09T19:48:05Z | |
| dc.date.issued | 2022-11-06 | |
| dc.description.sponsorship | Background: Because of the increased resistance of certain bacterial and fungal species to various antibiotics, uses of natural base chemicals such as honey and medicinal plants have become increasingly appealing. Honey is one of the most well-known natural antibacterial sources documented in ancient medicine. Objectives: To assess the antibacterial effects against Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Salmonellae typhimurium, and antifungal effects against Candida albicans of four Palestinian honey from different botanical origin and determine the antibacterial and antifungal effects of flower botanical origin for honey. The following honey samples will be assessed A. (Ziziphus) B. (Thymus vulgaris) C. (Citrus honey) D. (Wildflower honeys). Methodology: The antibacterial and antifungal effects were measured through assessment of the honey and plant antibacterial effect through; the disc diffusion method which is concerned as the main qualitative test for detecting the susceptibility of bacteria and fungi to antimicrobial substances, broth microdilution assay to determine minimum inhibitory concentration (MIC) assay, which reflects the quantity needed for bacterial and fungal inhibition, and minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) which reflects the quantity needed for of bacterial and fungal death respectively. Results: The present study reveals that local Palestinian honey that differ in botanical origin such as Sidr, Thymus, Multiflowers and Citrus honeys were effective in inhibiting the in vitro growth of Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Salmonellae typhimurium. Multiflowers honey was most potent than other honeys in inhibiting Klebsiella pneumoniae and Staphylococcus aureus growths where the mic50 values was 177.86±9.75 and 78.08±23 respectively and also Citrus honey was most potent than other honeys in inhibiting Escherichia coli and Salmonellae typhimurium growths and the mic50 values was 196.72±6.26 and 166.13±2.85 respectively. In vitro all honey samples in the different concentrations were more effective against Staphylococcus aureus than the other bacteria. There is no effect of the honeys on Candida albicans. Conclusion: According to the present results, the antibacterial activity of Palestinian honeys depends on their botanical origin, honey can be applied as antimicrobial agent against specific pathogenic bacteria, and the proliferation of bacterial strains is inhibited by these honeys. Keywords: Honey, Bacteria, Fungi, Botanical origin, Palestine. | |
| dc.identifier.uri | https://hdl.handle.net/20.500.11888/19019 | |
| dc.language.iso | en | |
| dc.supervisor | Prof. Raed Alkowni Prof. Hazem Sawalha | |
| dc.title | ANTIMICROBIAL EFFECT OF PALESTINIAN HONEY FROM DIFFERENT BOTANICAL ORIGIN AGAINST HUMAN PATHOGENIC BACTERIAL AND FUNGAL | |
| dc.type | Thesis |