ORIGANUM SYRIACUM AND ALOYSIA CITRODORA ESSENTIAL OIL: CHEMICAL PROFILE, ANTIBACTERIAL, ANTIOXIDANT AND ANTICANCER ACTIVITY
| dc.contributor.author | Lina Tayseer Hamdan | |
| dc.date.accessioned | 2024-05-30T12:13:41Z | |
| dc.date.available | 2024-05-30T12:13:41Z | |
| dc.date.issued | 2022-11-21 | |
| dc.description.abstract | Abstract Objectives: The belief that some plants have the power to treat many illnesses without side effects is one of the reasons why interest in medicinal plants is increasing day by day. In our area, two common plants Origanum syriacum "Za'atar" and Aloysia citrodora have long been employed in numerous traditional treatments. This study aims to explore the chemical profiles of the essential oils (EO) of Origanum syriacum collected in Jerusalem and Aloysia citrodora gathered in Jericho and Qalailya, as well as their antioxidant, anti-microbial, and anticancer properties. Studying the synergistic impact of their combination on cell proliferation of various cancer cell lines is another goal. Materials and methods: Using hydro distillation extraction, Origanum syriacum EO and Aloysia citrodora EO were produced, and the chemical components of the EO were identified qualitatively and quantitatively using GC-MS. By inhibiting DPPH free radicals, the antioxidant activity of the EOs was evaluated. The MTS assay was used to examine the anticancer activity. The micro-dillution method was used to test the antibacterial activity. Results: 11 compounds were identified in O. syriacum EO, Carvacrol(79.46%), thymol (15.87%) and cuminol(3.25 %) were the major components. 37 compounds found in A. citrodora EO from Jericho, the most abundant compounds were α-curcumene(26.94%), spathulenol(13.69%), geranial(10.79%), caryophellene oxide(8.66%), neral(7.59%) and ẞ-caryophyllene(6.14%); whearas 31 compounds identified in A. citrodora EO from Qalqilya, geranial(37.00%), neral(29.00%), α-curcumene(7.76%), ẞ-caryophyllene (6.00%), and bicyclogermacrene(2.79%) were the main constituents. The IC50 value of O. syriacum was 9.29±0.52 µg/mL while A. citrodora IC50 was 31.35±0.33 µg/mL. IC50 values of A. citrodora EO against the cancer cell lines were between 13.5±1.41 and 87.6±3.17 µg/mL, while O. syriacum EO ranging from 32.5±1.20 ̶ 84.9±3.41 µg/mL. The MIC values for O. syriacum EO ranged from 48.7 ̶ 25000 µg/mL, whereas those for A. citrodora EO were between 3125 and 10000 µg/mL. Conclusions: The antioxidant and antibacterial activity of O. syriacum exceeded that of A. citrodora EO. A. citriodora EO had more growth-inhibitory effects on all cell lines than O. syriacum EO. Their mixture (1:1 w: w) doesn’t show an enhancement on the anticancer activity. Keywords: Aloysia citrodora essential oil Antibacterial; Anticancer; Antioxidant; Chemical profile; Origanum syriacum essential oil; Synergistic effect. | |
| dc.identifier.uri | https://hdl.handle.net/20.500.11888/18903 | |
| dc.language.iso | en | |
| dc.supervisor | Dr. Nawaf Al-Maharik Dr. Nidal Jaradat | |
| dc.title | ORIGANUM SYRIACUM AND ALOYSIA CITRODORA ESSENTIAL OIL: CHEMICAL PROFILE, ANTIBACTERIAL, ANTIOXIDANT AND ANTICANCER ACTIVITY | |
| dc.type | Thesis |