INCORPORATING DIFFERENT LEVELS OF ADENOSINE WITH TRIS- EGG YOLK AND ANDROMED®DILUENTS USED IN FREEZING SEMEN OF RAMS

Simple item page
dc.contributor.authorKhaleel , Mousa
dc.date.accessioned2025-03-25T09:41:10Z
dc.date.available2025-03-25T09:41:10Z
dc.date.issued2025-01-30
dc.description.abstractBackground: Cryopreservation of ram semen is a pivotal technique in ovine genetic improvement strategies. It accelerates the transmission of superior genetic material to low-productivity flocks, enables the long-term preservation of valuable genetic resources, and mitigates geographical constraints in artificial insemination programs. However, ovine semen cryopreservation still faces challenges related to quality, as ram sperm cells are particularly vulnerable to thermal stress and oxidative damage during freezing. Adenosine, a purine nucleoside, has been shown to enhance cellular metabolism and antioxidant defense; however, its potential application in ovine semen cryopreservation remains underexplored. Aims: This study aims to elucidate the protective effects of adenosine at different concentrations (0%, 0.5%, 0.75%, and 1%) on sperm motility, viability, thermal resistance, membrane integrity, and morphology throughout the freezing process. Additionally, the study compares the efficacy of Andromed® and Tris-egg yolk extenders in maintaining sperm quality. Methods: Semen was collected from six mature Assaf rams aged 3 to 4 years and diluted with adenosine-enriched Andromed® and Tris-egg yolk diluents. Semen samples were evaluated for motility, viability, thermal resistance, membrane integrity, and morphology at three key stages of the freezing process. The data were statistically analyzed using ANOVA to evaluate the effects of adenosine concentration and extender type. Results: Adenosine significantly improved sperm quality at all freezing stages (P < 0.05), with 0.75% yielding the most favorable results. Moreover, Andromed® outperformed Tris-egg yolk in preserving frozen ovine sperm quality (P < 0.05). Conclusion: The incorporation of adenosine into semen extenders, particularly at 0.75%, significantly enhanced the quality of frozen ovine semen. Furthermore, Andromed® proved to be more effective than Tris-egg yolk as freezing extender. These findings provide valuable insights into the application of adenosine in cryopreservation and contribute to improved reproductive efficiency in ovines.
dc.identifier.urihttps://hdl.handle.net/20.500.11888/19999
dc.language.isoen
dc.publisherAn-Najah National University
dc.supervisorAbdallah , Jihad
dc.titleINCORPORATING DIFFERENT LEVELS OF ADENOSINE WITH TRIS- EGG YOLK AND ANDROMED®DILUENTS USED IN FREEZING SEMEN OF RAMS
dc.title.alternativeدمج تراكيز مختلفة من مركب الأدينوسين مع محاليل التمديد تريس-صفار البيض والاندروميد المستخدمة في تجميد السائل المنوي للكباش
dc.typeThesis
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Natural Sciences