Brugia Malayi: Depletion of Glutathione by Buthionine Sulfoximine

dc.contributor.author	Ayman Hussein
dc.date.accessioned	2016-09-07T10:16:50Z
dc.date.available	2016-09-07T10:16:50Z
dc.date.issued	2005
dc.description.abstract	Glutathione is an intracellular reducing agent. It is synthesized by a two-step reaction catalyzed by ?-glutamylcysteine synthetase (?GCS) and glutathione synthetase. ?GCS, which is the rate limiting enzyme in the synthesis of glutathione, is inhibited specifically by buthionine sulfoximine (BSO). The enzyme was partially purified from the parasitic nematode Brugia malayi, the causative agents of lymphatic filaraiasis. BSO inhibits the enzyme activity in an irreversible manner. The Brugia enzyme was found to be 24-fold more sensitive to BSO than its mammalian counterparts. The inhibitor was also found effective in depleting the glutathione contents of the parasites with low concentration. The results may conclude that ?GCS is a potential target for the development of drugs against filariasis.	en
dc.identifier	1727-2114
dc.identifier.uri	http://hdl.handle.net/20.500.11888/1983
dc.title	Brugia Malayi: Depletion of Glutathione by Buthionine Sulfoximine	en

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