Characterization of Polyphenol-Containing Polar Extracts from Stachys Palaestina and Stachys Viticina and Evaluation of Their Pharmacological Properties

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بدوان, ليلى محمد عباس
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An-Najah National University
The aromatic plant Stachys viticina (Lamiaceae) is a perennial herb growing in the Mediterranean countries including Palestine. And Like other Stachys species, it’s also used in folk therapy from ancient time. In view of this, the current work designed to isolate and characterize the chemical constituents and to assess the in-vitro, antioxidant, antimicrobial properties of the polar poly phenolic composition of Stachys viticina. Methanol extract of Stachys viticina was subjected to a sequence of silica gel column chromatography using different eluents with various polarities. The purity of the isolated fractions was conducted by thin layer chromatography (TLC) and confirmed using high performance liquid chromatography (HPLC). Moreover, functional groups of the pure fractions were detected using infrared spectroscopy. In addition, the radical scavenging capacity of plant methanolic extracts was assessed by the DPPH assay, and antimicrobial properties against seven microbial strains using the microdilution method were also screened. Seven extracts of Stachys viticina were separated (A-F and R). All of the extracts (A-D, F, and R) exhibited antioxidant activity with IC50values of 85.88 µg/mL, 54.37 µg/mL, 77.58 µg/mL, 68.36 µg/mL, 58.62 µg/mL, and 18.58 µg/mL, respectively. Fraction C had the highest antibacterial activity at a minimum concentration (39 µg/mL) against methicillin-resistant staphylococcus aureus (MRSA) and Staphylococcus aureus. But all of the extracts had no activity against candida albicans fungi strain.