DEVELOPMENT OF NON-COVALENTLY FUNCTIONALIZED MULTIWALLED CARBON NANOTUBES BASED-SUBSTRATE FOR SUPPORTING IN VITRO NEURONAL GROWTH
| dc.contributor.author | Rama Sahem Hendawi | |
| dc.date.accessioned | 2024-08-21T08:05:19Z | |
| dc.date.available | 2024-08-21T08:05:19Z | |
| dc.date.issued | 2023-06-22 | |
| dc.description.abstract | Abstract Background: Nerve injuries are considered the first leading cause of disabilities and one of the leading causes of deaths globally, which add a huge burden on socioeconomics. Many interventions have been developed to treat nerve injures, the golden standards are neurorrhaphy when suturing is possible, and autologous nerve transplantation, although promising results were obtained, functional recovery from chronic injuries remains a challenge. As a result, nerve tissue engineering emerged to find innovative solutions. In this study, the researcher aimed to develop neuronal growth by enhancing multiwall carbon nanotubes (MWCNT) dispersion and use it as a substrate. Objectives: This study aimed to test the effect of CNT substrate on neuronal functions and morphology, by first enhancing the MWCNT dispersion and form a substrate allows neuronal growth. Methods: in order to enhance MWCNT dispersion, the researcher functionalized it with poly-l-lysine (PLL) and chitosan by sonication, the cells were primary isolated from cortical rat embryo and seeded on PDMS polymer, and then we analyzed the substrate was analyzed by Raman spectra and scanning electronic microscope (SEM). Then we tested neuronal synchronicity was tested by calcium dye staining florescence, the recorded videos were further analyzed by SyincAnalysis software, the cellular viability, and synchronicity percentages were statistically analyzed by student T test. The neuronal morphology was analyzed by staining actin and tubulin, the main cytoskeletal filaments engaged in neuritogenesis, the recorded images were further analyzed by special software, and the results were statistically analyzed by one way ANOVA. Results and conclusions: PLL enhanced MWCNT dispersion better than chitosan, with 0.05% PLL-MWCNT as our working concentration. For neuronal synchronicity the researcher tested the substrate against PLL-ECM as a control, and it was that there is found no significant difference between active cell number percentage and synchronicity cell percentage which indicates the substrate ability to sustain neuronal activity. Furthermore, we tested neurite branching against PLL and PLL-ECM as controls, and we found significant difference between PLL-MWCNT and others, we hypothesized this increase due to nanoscale topography that are in the favorable size for focal adhesion attachment, and that PLL-MWCNT increased substrate stiffness. Keywords: Carbon nanotubes; poly-l-lysine; chitosan; synchronization; neuritogensis; nerve tissue engineering; substrate. | |
| dc.identifier.uri | https://hdl.handle.net/20.500.11888/19414 | |
| dc.language.iso | en | |
| dc.supervisor | Dr. Naim Kittana Dr. Mohyeddin Assali | |
| dc.title | DEVELOPMENT OF NON-COVALENTLY FUNCTIONALIZED MULTIWALLED CARBON NANOTUBES BASED-SUBSTRATE FOR SUPPORTING IN VITRO NEURONAL GROWTH | |
| dc.type | Thesis |