BIOLOGICAL AND GENETICAL INVESTIGATION FOR CONSERVATION OF PALESTINIAN IRIS HAYNEI OF FAQQUA
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Date
2022-09-25
Authors
Amer Barahmeh
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Abstract
Background:In Palestine, one of the symbolic flowering plants is Iris haynei. It is called "royal iris" and it's limited to the northeast of West Bank, particularly the village of Faqqua.
Aim:The main aim of this study was to better understand the current status of Iris haynei population to ensure their continuity in the Palestinian ecosystems.
Materials and method:Field surveys were conducted for some species to localize every plant on site and to check the fructification period (in-situ techniques). Past transplantation was monitored on-site and manual pollination was conducted and tested. On the other hand, seeds of the selected species were collected for the seed bank for the development of germination protocols (ex-situ techniques). Finally, after the lab studies have been conducted, the plants generated are ready to reinforce their original populations in their natural habitat (in-situ techniques). Besides, this study investigated the morphological and genetic variations of the planted Iris. For that, several Iris haynei species were grown in Faqqua Palestine and experiments were done including seed germination, genetic variation and site environmental scanning.
Results: In lab seed germination, 50% of seeds have been germinated in the presence of Gibberellic acid, while without hormone 34%. In the field, in the first year, none of the seeds have grown, while in the second year, 25% have grown. In" this study, the RAPD method was used for genotypes identification and characterization of "three Iris haynei colors (yellow, purple and pink). Five out of ten primers were tested for their ability to generate polymorphism from selected Iris haynei using RAPD-PCR, and the obtained data were analyzed. The similarity value analysis was 0.46 between (yellow, purple) Irises, while the similarity value between (yellow, pink) was 0.33. By this study, genetic diversities of studied were Iris haynei colored species ascertained successfully using RAPD markers, concluding that it could be a useful tool for identifying Iris species.
Keywords: ecosystem; Iris haynei; royal iris, RAPD.