FUNCTIONALIZATION OF ANTICANCER NATURAL COMPOUNDS (CITRONELLOL, P-COUMARIC ACID, CIS-JASMONE, AND THYMOL): PREPARATION, CHARACTERIZATION, AND CYTOTOXIC ACTIVITY
| dc.contributor.author | Nehaya Mohammed Yousef Salameh | |
| dc.date.accessioned | 2024-09-23T19:19:06Z | |
| dc.date.available | 2024-09-23T19:19:06Z | |
| dc.date.issued | 2024-03-09 | |
| dc.description.abstract | Abstract Functionalizing of natural compounds exhibiting biological activity is one of the most efficient approaches for the synthesizing biologically active substances to improve their pharmacological effects or to investigate new therapeutic approaches. This study aims to prepare new derivatives of bioactive compounds including citronellol, p-coumaric acid, cis-jasmone, and thymol by modification of their functional groups and to test their new biological activities whether there is an improvement or decline in these activities. In our investigation, we follow the methods of preparing new compounds such as esters, amides, anhydrides and amines through condensation reactions of alcohols with carboxylic acids, and through Michael’s addition methods. The new compounds were identified by FT-IR and NMR analysis. MTS assay was used to evaluate their anticancer activity. The broth dilution method was used to assess their antimicrobial activity. Some of the new derivatives of natural products showed anticancer and antimicrobial activities more potent than the starting natural compound and others showed lower potency than the starting natural compound that was used to prepare new derivatives, but better preserving normal cell line. The newly synthesized derivatives were successfully prepared, and their structures were verified by spectroscopic analysis. Their biological activities were tested against different cancer lines and microbial strains. Among the citronellol esters: - J15 was the most potent against liver cancer Hep-G2. The p-CA ester J14 was the most potent anticancer agent against CaCo-2, Hep-G2, HeLa, and MCF-7 cancer cell lines, J14 also showed distinctively higher activity against all microorganisms except Pseudomonas aeruginosa. The p-CA amide J19 showed antimicrobial activity against Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Candida albicans. The p-CA anhydride N2 was the most potent against Hep-3B and MCF-7 cell lines while N4 was the most potent against HeLa cell lines. Among the cis-jasmone amines, N6 was the most potent against HeLa, and B16-F1cell lines. The amine N8 showed distinctively highest antimicrobial activity against Klebsiella pneumoniae, P. vulgaris, P. aeruginosa, and C. albicans. The thymol ester J16 was the most potent against CaCo-2, Hep-G2, HeLa, MCF-7, and skin cancer B16-F1 and showed distinctively highest activity against all microorganisms except against E. coli J12 was the most potent. Keywords: Natural anticancer compounds, Citronellol, Thymol, cis-Jasmone, p-CA. | |
| dc.identifier.uri | https://hdl.handle.net/20.500.11888/19550 | |
| dc.language.iso | en | |
| dc.supervisor | Prof. Mohammed Al-Nuri Prof. Nidal Jaradat | |
| dc.title | FUNCTIONALIZATION OF ANTICANCER NATURAL COMPOUNDS (CITRONELLOL, P-COUMARIC ACID, CIS-JASMONE, AND THYMOL): PREPARATION, CHARACTERIZATION, AND CYTOTOXIC ACTIVITY | |
| dc.type | Thesis |