Characterization of Bioactive Phytochemicals from Micromeria Fruticosa Plant as an Approach to Develop Natural-based Drug Leads

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Alhajeh, Anwar Afeef
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جامعة النجاح الوطنية
Four parts (flowers, leaves, stems, roots) from Micromeria Fruticosa plant used in traditional medicine in Palestine were tested for their biological activity against four types of bacteria and one type of fungi {S. sonnie (ATCC 25931), S. aureus (ATCC 25923), Escherichia coli (E. coli), MARSA, and Candida albicans (C. albicans) (ATCC 90028)} respectively. The plant was collected from Tulkarem region. The family name of plant is Micromeria Fruticosa (L) Druce ssp serpyllifolia (Lamiaceae) and is known as ashab a-shai in Arabic. The ethanolic, hexane and distilled water extracts of those parts of the plants were tested for their antioxidant activity through DPPH assay. The D.W. extract from leave has got the highest percentage inhibition (89%) at the concentration (100µg/ml). Some constituents were detected from ethanolic and hexane plants extracts using GC-MS/MS spectrophotometer and separated by flash chromatography the most similar compound found in all part in plant is 9-OCTADECENAMIDE, (Z)-, Menthol and CYCLOHEXANONE,5-METHYL-2-(1-METHYLETHYL)-, TRANS with different high, and area percentage. Also, some elements were detected from distilled water plant extracts using ICPMS analysis, these elements are Fe, Zn, Sr with different percentage per extract part of plant.