Anti-Inflammatory Effects Of Herbal-Derived Factors Are Mediated By Down Regulation Of Pro-Inflammatory Cytokines
Date
2010-09-26
Authors
Bahaa Hadieh
Basher Abo Farich
Omar Said
Bashar Saad
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
<p>The aim of the present in vitro study is to evaluate the role of pro-inflammatory cytokines<br />
tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) in the observed anti-inflammatory<br />
effects of Hypericum triquetrifolium and Peganum harmale. Therefore, water extracts from<br />
the aerial parts of the two plants were tested for their anti-inflammatory effects in vitro using<br />
cells from the human monocyte cell line THP-1. Anti-inflammatory effects were assessed by<br />
measuring the levels of secretory proteins and mRNA of TNF-α and IL-6 production of LPSactivated<br />
THP-1 cells. Cells were treated with 5μg lipopolyssacharide/ml (LPS) in the<br />
absence and presence of increasing concentrations of plant extracts. During the entire<br />
experimental period, we used plant extracts concentrations (up to 250μg/ml) that had no<br />
cytotoxicity as measured in MTT assay. Hypericum triquetrifolium extracts totally reduced<br />
the LPS induced TNF-α expression and secretion. Peganum harmale extracts inhibited the IL-<br />
6 production in a dose dependent manner reaching the control levels of untreated cells at a<br />
concentration of 125 μg/ml. These results demonstrate that the anti-inflammatory effects of<br />
the two plants are mediated through the inhibition of the pro-inflammatory cytokines TNF-α<br />
and IL-6.</p>
<p>The aim of the present in vitro study is to evaluate the role of pro-inflammatory cytokines<br /> tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) in the observed anti-inflammatory<br /> effects of Hypericum triquetrifolium and Peganum harmale. Therefore, water extracts from<br /> the aerial parts of the two plants were tested for their anti-inflammatory effects in vitro using<br /> cells from the human monocyte cell line THP-1. Anti-inflammatory effects were assessed by<br /> measuring the levels of secretory proteins and mRNA of TNF-α and IL-6 production of LPSactivated<br /> THP-1 cells. Cells were treated with 5μg lipopolyssacharide/ml (LPS) in the<br /> absence and presence of increasing concentrations of plant extracts. During the entire<br /> experimental period, we used plant extracts concentrations (up to 250μg/ml) that had no<br /> cytotoxicity as measured in MTT assay. Hypericum triquetrifolium extracts totally reduced<br /> the LPS induced TNF-α expression and secretion. Peganum harmale extracts inhibited the IL-<br /> 6 production in a dose dependent manner reaching the control levels of untreated cells at a<br /> concentration of 125 μg/ml. These results demonstrate that the anti-inflammatory effects of<br /> the two plants are mediated through the inhibition of the pro-inflammatory cytokines TNF-α<br /> and IL-6.</p>
<p>The aim of the present in vitro study is to evaluate the role of pro-inflammatory cytokines<br /> tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) in the observed anti-inflammatory<br /> effects of Hypericum triquetrifolium and Peganum harmale. Therefore, water extracts from<br /> the aerial parts of the two plants were tested for their anti-inflammatory effects in vitro using<br /> cells from the human monocyte cell line THP-1. Anti-inflammatory effects were assessed by<br /> measuring the levels of secretory proteins and mRNA of TNF-α and IL-6 production of LPSactivated<br /> THP-1 cells. Cells were treated with 5μg lipopolyssacharide/ml (LPS) in the<br /> absence and presence of increasing concentrations of plant extracts. During the entire<br /> experimental period, we used plant extracts concentrations (up to 250μg/ml) that had no<br /> cytotoxicity as measured in MTT assay. Hypericum triquetrifolium extracts totally reduced<br /> the LPS induced TNF-α expression and secretion. Peganum harmale extracts inhibited the IL-<br /> 6 production in a dose dependent manner reaching the control levels of untreated cells at a<br /> concentration of 125 μg/ml. These results demonstrate that the anti-inflammatory effects of<br /> the two plants are mediated through the inhibition of the pro-inflammatory cytokines TNF-α<br /> and IL-6.</p>