Anti-Inflammatory Effects Of Herbal-Derived Factors Are Mediated By Down Regulation Of Pro-Inflammatory Cytokines

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dc.contributor.authorBahaa Hadieh
dc.contributor.authorBasher Abo Farich
dc.contributor.authorOmar Said
dc.contributor.authorBashar Saad
dc.date.accessioned2017-05-03T09:36:07Z
dc.date.available2017-05-03T09:36:07Z
dc.date.issued2010-09-26
dc.description.abstract<p>The aim of the present in vitro study is to evaluate the role of pro-inflammatory cytokines<br /> tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) in the observed anti-inflammatory<br /> effects of Hypericum triquetrifolium and Peganum harmale. Therefore, water extracts from<br /> the aerial parts of the two plants were tested for their anti-inflammatory effects in vitro using<br /> cells from the human monocyte cell line THP-1. Anti-inflammatory effects were assessed by<br /> measuring the levels of secretory proteins and mRNA of TNF-α and IL-6 production of LPSactivated<br /> THP-1 cells. Cells were treated with 5μg lipopolyssacharide/ml (LPS) in the<br /> absence and presence of increasing concentrations of plant extracts. During the entire<br /> experimental period, we used plant extracts concentrations (up to 250μg/ml) that had no<br /> cytotoxicity as measured in MTT assay. Hypericum triquetrifolium extracts totally reduced<br /> the LPS induced TNF-α expression and secretion. Peganum harmale extracts inhibited the IL-<br /> 6 production in a dose dependent manner reaching the control levels of untreated cells at a<br /> concentration of 125 μg/ml. These results demonstrate that the anti-inflammatory effects of<br /> the two plants are mediated through the inhibition of the pro-inflammatory cytokines TNF-α<br /> and IL-6.</p>en
dc.description.abstract<p>The aim of the present in vitro study is to evaluate the role of pro-inflammatory cytokines<br /> tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) in the observed anti-inflammatory<br /> effects of Hypericum triquetrifolium and Peganum harmale. Therefore, water extracts from<br /> the aerial parts of the two plants were tested for their anti-inflammatory effects in vitro using<br /> cells from the human monocyte cell line THP-1. Anti-inflammatory effects were assessed by<br /> measuring the levels of secretory proteins and mRNA of TNF-α and IL-6 production of LPSactivated<br /> THP-1 cells. Cells were treated with 5μg lipopolyssacharide/ml (LPS) in the<br /> absence and presence of increasing concentrations of plant extracts. During the entire<br /> experimental period, we used plant extracts concentrations (up to 250μg/ml) that had no<br /> cytotoxicity as measured in MTT assay. Hypericum triquetrifolium extracts totally reduced<br /> the LPS induced TNF-α expression and secretion. Peganum harmale extracts inhibited the IL-<br /> 6 production in a dose dependent manner reaching the control levels of untreated cells at a<br /> concentration of 125 μg/ml. These results demonstrate that the anti-inflammatory effects of<br /> the two plants are mediated through the inhibition of the pro-inflammatory cytokines TNF-α<br /> and IL-6.</p>ar
dc.identifier.urihttps://hdl.handle.net/20.500.11888/9330
dc.titleAnti-Inflammatory Effects Of Herbal-Derived Factors Are Mediated By Down Regulation Of Pro-Inflammatory Cytokinesen
dc.titleAnti-Inflammatory Effects Of Herbal-Derived Factors Are Mediated By Down Regulation Of Pro-Inflammatory Cytokinesar
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Second conference on Biotechnology Research and Application In Palestine