Extraction, Characterization and Application of Essential oil from Native Palestinian Plants
Date
2017-06-06
Authors
ابوالرب, منى
Journal Title
Journal ISSN
Volume Title
Publisher
جامعة النجاح الوطنية
Abstract
In this work, the effect of the extracted oils from three different plants(Oleander, Rutagraveolens, and Basil ) were studied against insects such as Sitophilus granaria, Mosquito, and Varroa mites as it has been noticed recently that there's an increase in the use of chemically manufactured pesticides, which contributed to the pollution of the environment and human health. It's important to say that these extracted oils have some insecticidal activities.
These plants (Oleander, Rutagraveolens, and Basil) were selected since there are some previous studies showing activity of these extracts against some diseases, and showing antimicrobial and fungicidal activities. Therefore, in this study, the extracted oils have been tested as insecticides and insect repellent. They have been applied to Sitophilus granaria, Mosquito, and Varroa mites as these insects have harmful effects on the health and economy of human being.
The plants were collected from the NARC Center where they were collected through the flowering process and then taken to the laboratory to extract the oils. 800 g of each plant was taken and cut into small pieces , soaked with ethanol for 48 hours and then the solvent was filtered and evaporated to get the extracted oil. Both TLC and GC-MS analysis were performed in order to identify the organic compounds.
In addition, The essential oil from these plants were tested against the above insects, obtained from the Faculty of Agriculture at An-Najah University. The extracted oils were tested as repellents for the Sitophilus granaria by placing them on filter-filled paper and leaving the paper slightly in the air to evaporate the solvent and then put the insects on it. These oils were then tested if they have some biocidal activity by the vaporization process.
The GC-MS analysis showed that the oleander and basil had a common compound number ( VI )* which had insecticidal activity. Furthermore, the Ruta and Oleander had a common compound called (I)*. From the test conducted on the application of extracts, it was observed that oleander had 100% repellant activity against Sitophilus granaria followed by Ruta (98%), and Basil (95%) respectively. On the other hand, it was observed that of Oleander and Ruta essential oils showed the highest percent mortality against mosquito with nearly 100% mortality rate. However, the Basil had the highest mortality rate ( 80%) against mosquito. Other insects had lower mortality rates compared to the previous results. Further studies should be conducted in order to isolate and characterize the efficacy, the toxicity and the efficiency of the active ingredients as natural sources of insecticides.