Effect of New Copper Complexes on Cancer Cells in Inducing Caspase-Dependent Apoptosis and Binding to DNA
Shana’a, Jihad Sameeh Shafeeq
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Cancer spread worldwide as one of main reasons for mortality. There are more than 100 types of cancer. There were 14.1 million new cancer cases in 2012. By year 2030 new cases will reach to 21.7 million due to modernized lifestyles (such as smoking, poor diet, physical inactivity, and fewer pregnancies … etc.) that are known to increase cancer risk. Cancer treatment options may include chemotherapy, radiation, and/or surgery or a combination of all. Chemotherapy is one of the major treatment options that are available to face this problem. One of the most noteworthy discoveries in cancer chemotherapy is cisplatin, which is very active against different types of cancer. Cisplatin has a cure rate exceeded 90% in some cancer types. However, the treatment option by using cisplatin is limited by its side effects, also the inherited and acquired resistance limits its usage too. These obstacles have led to the extensive search for other active antitumor metal-based complexes with improved pharmacological properties. This research was aiming to study the anticancer activity of copper complexes that depended on activating apoptosis through caspases pathway. Nine copper-based complexes were prepared and screened in-vitro for cytotoxic activities against three cancer cell lines (Colon cancer: colo205 cell line, Osteosarcoma cell line: Hs 755 and Breast cancer cell line: MC7 cell line) using caspase-3 colorimetric assay and cell viability test (MTT assay). Results showed that complexes 1,3,4,5,6 and complex 8 induced cytotoxic effect on colo205, while complex 2 and 7 have no cytotoxic effect on colon cancer cells. All the complexes that tested didn’t show any sign of activating caspase 3 for Colon cell line or Breast cancer cell line MC7. These complexes exhibited promising anti-proliferative activity against tested cell lines, which indicates that these complexes have anticancer effects.