Second conference on Biotechnology Research and Application In Palestine
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- ItemAnti-Inflammatory Effects Of Herbal-Derived Factors Are Mediated By Down Regulation Of Pro-Inflammatory Cytokines(2010-09-26) Bahaa Hadieh; Basher Abo Farich; Omar Said; Bashar Saad
The aim of the present in vitro study is to evaluate the role of pro-inflammatory cytokines
tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) in the observed anti-inflammatory
effects of Hypericum triquetrifolium and Peganum harmale. Therefore, water extracts from
the aerial parts of the two plants were tested for their anti-inflammatory effects in vitro using
cells from the human monocyte cell line THP-1. Anti-inflammatory effects were assessed by
measuring the levels of secretory proteins and mRNA of TNF-α and IL-6 production of LPSactivated
THP-1 cells. Cells were treated with 5μg lipopolyssacharide/ml (LPS) in the
absence and presence of increasing concentrations of plant extracts. During the entire
experimental period, we used plant extracts concentrations (up to 250μg/ml) that had no
cytotoxicity as measured in MTT assay. Hypericum triquetrifolium extracts totally reduced
the LPS induced TNF-α expression and secretion. Peganum harmale extracts inhibited the IL-
6 production in a dose dependent manner reaching the control levels of untreated cells at a
concentration of 125 μg/ml. These results demonstrate that the anti-inflammatory effects of
the two plants are mediated through the inhibition of the pro-inflammatory cytokines TNF-α
and IL-6. - ItemMolecular Characterization and Detection of Redglobe-Isolated Closterovirus(2010-09-26) Raed Alkowni; Yun-Ping Zhang; Jerry K. Uyemoto; Angelantonio Minafra; Deborah Golino; Adib Rowhani
- ItemPalestinian Herbal Plant Increases glucose disposal by skeletal muscle cell line(2010-09-26) Hilal Zaid; Omar Said; Bashar Saad
- ItemThe genetic basis of plant water use efficiency(2010-09-26) M.V. Mickelbart
- ItemHuman cytomegalovirus replication cycle is regulated by hepatocyte growth factor/c-met pathway(2010-09-26) Nashat Al-Jalad; Mahmoud Al-Saheb; Yaqoub Ashhab
- ItemFactors involved in the early events of spore germination and host colonization by Botrytis cinerea(2010-09-26) Salem Nassr; Radwan Barakat
- ItemMolecular-based identification of six strains of entomopathogenic fungi(2010-09-26) Isra’ Al_qadi; Iba’ Farrah; Naim Iraki
- ItemVitamin B12 Deficiency among Palestinians: A Critique Study(2010-09-26) Khalid Younis
In fact, it takes several years for a person to develop vitamin B12 deficiency as a result ofmalabsorption because the daily requirement of vitamin B12 is low (around 3 mg) and thestorage rate is high (around 5 mg). Also, it is well known that the methods used to determineVit B12 do lack specificity (50%) and sensitivityn(95%). Based on the available data, 25%-50% of the time there were low levels of vitamin B12. As low levels of vitamin B12 withoutvitamin B12 deficiency have been reported, are all the individuals in the study with low levelsof vitamin B12 truly having vitamin B12 deficiency? Conversely, some individuals withnormal levels of Vit B12 do have magaloblastic anemia. As each method of assay has its owndrawbacks (CV is around 11% and they lack sensitivity and specificity), and vitamin B12deficiency may be identified in the absence of the expected clinical and hematologic findingsand visa versa, a careful and intelligent interpretation of results is very essential. To confirmlow or suspected results of vitamin B12, it is recommended to test also for serum and red cellfolate, serum homocystein, and urine MMA.An isolated result of serum B12 should not be the sole criterion on which treatment decisionis based, and a repeat assay and other confirmatory and clinical evaluation are necessary priorto a diagnostic conclusion. Additional secondary testing with metabolite levels andmonitoring of treatment response is recommended.Accordingly, I think that the high incidence of Vit B12 deficiency among Palestinians Is Fakebecause of misinterpretation.
- ItemIn vitro Propagation and the Production of Secondary Metabolites from Arbutus andrachne L(2010-09-26) Zahra Al-Jabary; Rami Arafeh
- ItemPartial sequence of the Erysiphe necator cytochrome b gene(2010-09-26) Hajaj Hajjeh; Monica Miazzi; Franco Faretra
- ItemBiotechnological and chemo diversity approaches of Plant secondary metabolites(2010-09-26) Khalid Sawalha
Plant secondary metabolites are evaluated as strong potential for biotechnologicalapplications. Plants are well known of arsenal chemical defense against pathogens, herbivoresand pests. Theses active compounds are chemically diverse and categorized according to theirstructures reflecting wide spectrum of phyto-chemicals. Among the most active compoundsproduced by plants are: alkaloids, essential oils, flavonoids, glucosinolates and phenols.Although these compounds are used by plants in their ecological interactions, however;secondary compounds are used as major sources of alternative medicines, bio-pesticides, foodadditives or in cosmetic industry. These benefits can be achieved through biotechnologicalapproaches for example plant tissue culture and genetic engineering.
- ItemCloning and Expression of a Bacteriophage DNA Ligase for molecular cloning(2010-09-26) Alia’ AL-Manasra; Fawzi Alrazem
- ItemEffect of Carob (Ceratonia siliqua L) male flower on the ovulation in mice(2010-09-26) Hadil Jaber; Walid Basha; Nidal Jaradat; Maan Sharaf
The results showed a significant effect on the ovulation in mice. More researchs are in process
to identify the specific effect on the hormonal secretion in mice. A future research on human
could help in giving a promising treatment for infertility in human. - ItemUsing Computer Vision to Cluster Plants based on Compound Leaf Morphology and Geometry(2010-09-26) Duaa Abu Maizer; Haneen Tartory; Asma Idais; Rami Arafeh; Hashem Tamimi
- ItemTowards restricting the spread of bacterial diseases in Palestine: Diagnosis of Agrobacterium tumefaciens in soil samples from stone fruit nurseries in three regions of the West Bank(2010-09-26) Osama Al-abdallah; Naim Iraki; Naser Jaghoub
Agrobacterium. tumefaciens is a soil borne pathogen infecting a wide range of cultivatedcrops and causes the crown gall disease (Jun Z et al 2000, Matthew A. E et al 2003) . Thisdisease causes a remarkable economic loss, especially when it affects trees (Kennedy B. etal1980) . A large part of the loss could be prevented if an appropriate phytosanitary strategiesare employed. Continuous testing of the cultivation medium and the seedlings for thepresence of this pathogen followed by an eradication of the infected material would have acrucial role in restricting the spread of this disease.Soil samples were collected from different commercial stone fruit nurseries located in threeregions; north, midwest, and south of the West Bank and they were tested for A.Tumefaciens. Aliquots of 100 μl from soil suspension were inoculated on semi-selectivemedia. Typical colonies developed on this selective medium were subjected to molecularbased diagnosis for A. tumefaciens using PCR techniques. The diagnosis employed specificprimers for the ipt gene located on the Ti plasmid (T-DNA) of the A. tumefaciens ( HaasJ.H. et al 1995, Cubero J. 1999 )A total of 200 samples from all areas were tested. In the North, 21 out of 129 samples werefound to be infected. In the South, 11 out of 59 samples showed infection withAgrobacterium. In the Midwest, however, only 12 samples were tested and only one of themwas infected. The percentage of infection is almost at the same level in the North and theSouth, 16 and 18%, respectively. Whereas, in the Midwest, the infection level was muchlower, but this value is not reliable due to the small number of tested samples.In conclusion, the results indicate a remarkable rate of infection with Agrobacteriumtumefaciens in commercial nurseries producing stone fruit seedlings. This implies that amore severe monitoring measures should be implemented by the Palestinian Ministry ofAgriculture to assure the use of certified plant sources and sterilized culture media.
- ItemIsolation of Putative Soil Streptomyces spp. Showing Antibacterial Activity(2010-09-26) Sameer Barghouthi; Saleh Abu-Lafi; Mohammed Ayesh; Samira Barghouthi; Rania Ghanem; Ashraf Zayed; Mohammed Qadi
- ItemUsing Genetic Programming to Design a New System that Enables Discovery of Novel Caspase3 Substrates(2010-09-26) Ala' Jabari; Hashem Tamimi; Yaqoub Ashhab
- ItemNano-sized metallic clusters for bio-applications(2010-09-26) Mohammed Suleiman
- ItemMutation in the 14α-Demethylase Gene of Erysiphe necator(2010-09-26) Hajaj Hajjeh; Monica Miazzi; Franco Faretra
- ItemBACTOSOM-Viewer: a High-Throughput Bacterial Clustering and Mapping Tool Based on Landmark Proteins(2010-09-26) Bilal Tamimi; Sami Salamin; Hashem Tamimi; Yaqoub Ashhab
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