Different methods were used for Sulfur nanoparticles (S NPs) synthesis; among those, chemical precipitation, electrochemical method, micro emulsion technique, composing of oil, surfactant, co-surfactant, aqueous phases with the specific compositions and ultrasonic treatment of sulfur-cystine solution. In this work S NPs were prepared by a quick precipitation method using sodium thiosulphate and tetraoctylammonium bromide surfactants in conc. hydrochloric acid media [1]. The sizes and shapes of S-NPs were confirmed by scanning electron microscope (SEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD) techniques.
Broth micro-dilution method was applied to determine antibacterial activity of S-NPs against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa reference strains. S-NPs exhibited antimicrobial activity (MIC = 5.47µg/mL) against Staphylococcus aureus strain (Gram-positive bacterium). On the other hand, no antimicrobial activity was detected against Gram-negative bacteria isolates (Escherichia coli and Pseudomonas aeruginosa) at0.68 to800 µg/mL.