Natural Sciences

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https://hdl.handle.net/20.500.11888/18293

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Browsing Natural Sciences by Author "Elsaid, Eman"

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    PHYTOCHEMICAL COMPOSITION AND ANTIDIABETIC, ANTI-OBESITY, ANTIOXIDANT, AND ANTIMICROBIAL ACTIVITIES OF SARCOPOTERIUM SPINOSIUM OIL
    (An-Najah National University, 2025-09-07) Elsaid, Eman
    Background: Obesity, diabetes, oxidative stress, and antimicrobial resistance are concerns negatively impacting individual health and represent an obstacle for the health care system. These problems drive researchers to discover novel solutions to prevent the impact of the issue from spreading worldwide. Medicinal plants are commonly used in traditional medicine to treat and prevent diseases. This study aims to investigate the antilipase, anti-amylase, antioxidant potential activity and antimicrobial activity against human pathogenic bacteria and phytopathogenic fungi of Sarcopoterium spinosum fixed seed oil. Additionally, phytochemical components of Sarcopoterium spinosum fixed oil extracted from seeds were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) techniques. Moreover, identified the phytopathogenic fungi by molecular techniques. Methods: Antioxidant activity was measured by using free radical scavenging activity, with DPPH reagent, and Trolox used as a reference. Although antilipase for S. spinosum oil potential was measured by measuring the amount of hydrolysis of p-nitrophenolate to p-nitrophenol were p nitrophenyl butyrate (PNPB) was utilized, and pancreatic lipase activity was estimated using a spectrophotometer at 405 nm. Orlistat medication was used as a reference. Where, antiamylase activity of S. spinosum fixed oil was evaluated by using DNSA reagents, and acarbose was used as a reference. Antimicrobial activity was performed against human pathogenic bacteria and phytopathogenic fungi by using disk diffusion methods for antibacterial and microbroth dilution methods assisted with dissecting microscopes for phytopathogenic fungi. Molecular identification of phytopathogenic fungi was conducted using PCR with ITS primers and sequencing. Results: S. spinosum oil with a composition of nine fatty acids showed significant antioxidant activity with IC50 value equal to 10.581 μg/ml, were strong α-Amylase Inhibitory potential with IC50 value 222.40 μg/ml, but no activity of pancreatic lipase enzyme. In addition, S. spinosum oil revealed no noticeable activity against bacteria, but activity against important Paecilomyces niveus, Penicillium expansum. Alternaria alstroemeria Botrytis californica, and Fusarium equiseti phytopathogenic fungi. Additionally, phytopathogenic fungi were identified by molecular methods. Conclusion: S. spinosum fixed seed oil has remarkable antioxidant properties and eco-friendly fungicide function against phytopathogenic fungi. Molecular techniques are effective in determining the precise organisms responsible for plants diseases.