Development of the bones and synovial joints in the rat model of the VATER association
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Date
2008-11-16
Authors
Prof. Ghassan Abu Hijleh
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Abstract
<p>The adriamycin-induced rat model of the Vertebral, Anorectal. Tracheo-Esophageal. Radial and Renal (VATER) association produces a variety of vertebral, rib, and limb abnormalities. This study was designed to document accurately the nature of these abnormalities and to determine whether synovial joints are affected. Fetuses from pregnant Sprague Dawley rats that had received intraperitonealinjections of 1.75 mg/kg of Adriamycin on days 6-9 or 10-13 of gestation were harvested. Doublestainedskeletal preparation and histological sections were examined for vertebral, rib, and limb anomalies. The incidence of abnormalities was high in the group treated on gestational days (GD) 6-9,while it was low in the GD 10-13 group. The length and thickness of the long bones were reduced, with bowing and reduction in their endochondral ossification. Sirenomelia occurred in the group treated onGD 6-9, and was often associated with a short tail and anal atresia. The joint cavities and intra-articularstructures such as menisci and the cruciate ligaments developed normally from the mesenchymalinterzone. These data indicate that Adriamycin inhibits skeletal growth and differentiation without any interference in the differentiation of the mesenchymal interzone, thus producing normal synovial joints.</p>
<p>The adriamycin-induced rat model of the Vertebral, Anorectal. Tracheo-Esophageal. Radial and Renal (VATER) association produces a variety of vertebral, rib, and limb abnormalities. This study was designed to document accurately the nature of these abnormalities and to determine whether synovial joints are affected. Fetuses from pregnant Sprague Dawley rats that had received intraperitonealinjections of 1.75 mg/kg of Adriamycin on days 6-9 or 10-13 of gestation were harvested. Doublestainedskeletal preparation and histological sections were examined for vertebral, rib, and limb anomalies. The incidence of abnormalities was high in the group treated on gestational days (GD) 6-9,while it was low in the GD 10-13 group. The length and thickness of the long bones were reduced, with bowing and reduction in their endochondral ossification. Sirenomelia occurred in the group treated onGD 6-9, and was often associated with a short tail and anal atresia. The joint cavities and intra-articularstructures such as menisci and the cruciate ligaments developed normally from the mesenchymalinterzone. These data indicate that Adriamycin inhibits skeletal growth and differentiation without any interference in the differentiation of the mesenchymal interzone, thus producing normal synovial joints.</p>
<p>The adriamycin-induced rat model of the Vertebral, Anorectal. Tracheo-Esophageal. Radial and Renal (VATER) association produces a variety of vertebral, rib, and limb abnormalities. This study was designed to document accurately the nature of these abnormalities and to determine whether synovial joints are affected. Fetuses from pregnant Sprague Dawley rats that had received intraperitonealinjections of 1.75 mg/kg of Adriamycin on days 6-9 or 10-13 of gestation were harvested. Doublestainedskeletal preparation and histological sections were examined for vertebral, rib, and limb anomalies. The incidence of abnormalities was high in the group treated on gestational days (GD) 6-9,while it was low in the GD 10-13 group. The length and thickness of the long bones were reduced, with bowing and reduction in their endochondral ossification. Sirenomelia occurred in the group treated onGD 6-9, and was often associated with a short tail and anal atresia. The joint cavities and intra-articularstructures such as menisci and the cruciate ligaments developed normally from the mesenchymalinterzone. These data indicate that Adriamycin inhibits skeletal growth and differentiation without any interference in the differentiation of the mesenchymal interzone, thus producing normal synovial joints.</p>