Prevalence of human papillomavirus and Chlamydia trachomatis infection in paired urine and cervical smear samples of Palestinian Young women
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Date
2010-10-20
Authors
Dr. Walid Basha
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Abstract
<p>Purpose:<br />
To investigate the prevalence and concordance of Human papillomavirus (HPV) and Chlamydia trachomatis (CT) in paired urine and cervical smear samples in Palestinian women.<br />
Methods:<br />
Cervical and urine specimens from 109 women (17-28 years old) visited governmental or private obstetrics and gynecology clinics for various reasons in the West Bank were randomly collected as a part of cohort study and stored at -20C ْ before phenol-chloroform DNA extraction were performed. All samples were tested using LCR-E7 PCR assay for detection HPV E6 and E7 genes and a new cryptic plasmid -PCR method for detection CT. Written agreement forms were received for participation in this project from all women.<br />
Results/findings:<br />
HPV DNA was detected in 6.4% (7/109) of cervical samples and 2.7% (3/109) of urine samples. 9.2% (10/109) of cervical samples and 6.4% (7/109) of urine samples were positive for CT; 0.9% (1/109) were positive for HPV or CT in urine samples alone.<br />
Conclusions:<br />
Urine samples can replace conventional cervix swabs for the detection of CT but not for HPV detection. The high prevalence illustrates the need for social education or prevention programs such as vaccination for these sexually transmitted infections should be considered in Palestine.</p>
<p>Purpose:<br /> To investigate the prevalence and concordance of Human papillomavirus (HPV) and Chlamydia trachomatis (CT) in paired urine and cervical smear samples in Palestinian women.<br /> Methods:<br /> Cervical and urine specimens from 109 women (17-28 years old) visited governmental or private obstetrics and gynecology clinics for various reasons in the West Bank were randomly collected as a part of cohort study and stored at -20C ْ before phenol-chloroform DNA extraction were performed. All samples were tested using LCR-E7 PCR assay for detection HPV E6 and E7 genes and a new cryptic plasmid -PCR method for detection CT. Written agreement forms were received for participation in this project from all women.<br /> Results/findings:<br /> HPV DNA was detected in 6.4% (7/109) of cervical samples and 2.7% (3/109) of urine samples. 9.2% (10/109) of cervical samples and 6.4% (7/109) of urine samples were positive for CT; 0.9% (1/109) were positive for HPV or CT in urine samples alone.<br /> Conclusions:<br /> Urine samples can replace conventional cervix swabs for the detection of CT but not for HPV detection. The high prevalence illustrates the need for social education or prevention programs such as vaccination for these sexually transmitted infections should be considered in Palestine.</p>
<p>Purpose:<br /> To investigate the prevalence and concordance of Human papillomavirus (HPV) and Chlamydia trachomatis (CT) in paired urine and cervical smear samples in Palestinian women.<br /> Methods:<br /> Cervical and urine specimens from 109 women (17-28 years old) visited governmental or private obstetrics and gynecology clinics for various reasons in the West Bank were randomly collected as a part of cohort study and stored at -20C ْ before phenol-chloroform DNA extraction were performed. All samples were tested using LCR-E7 PCR assay for detection HPV E6 and E7 genes and a new cryptic plasmid -PCR method for detection CT. Written agreement forms were received for participation in this project from all women.<br /> Results/findings:<br /> HPV DNA was detected in 6.4% (7/109) of cervical samples and 2.7% (3/109) of urine samples. 9.2% (10/109) of cervical samples and 6.4% (7/109) of urine samples were positive for CT; 0.9% (1/109) were positive for HPV or CT in urine samples alone.<br /> Conclusions:<br /> Urine samples can replace conventional cervix swabs for the detection of CT but not for HPV detection. The high prevalence illustrates the need for social education or prevention programs such as vaccination for these sexually transmitted infections should be considered in Palestine.</p>