Integrated Exposure Assessment of Sewage Workers to Genotoxicants: An Urinary Biomarker Approach and Oxidative Stress Evaluation

dc.contributor.authorHamzeh Al Zabadi
dc.date.accessioned2017-05-03T09:36:38Z
dc.date.available2017-05-03T09:36:38Z
dc.date.issued2011-05-04
dc.description.abstract<p>Background: Sewage workers are exposed to multiple chemicals among which many are suspected genotoxicants.Therefore, they might incur DNA damage and oxidative stress. We aimed to explore integrated biomarkers ofexposure and of early effects, respectively, among sewage workers, assessing the overall urine genotoxicity by invitro comet and micronucleus assays and measuring urinary 8-oxo-2'-deoxyguanosine (8-oxodG).Methods: During three consecutive working days, polycyclic aromatic hydrocarbons (PAHs) and volatile organiccompounds (VOCs) were sampled in workplace air of 34 sewage and 30 office workers, as indicators of airborneexposure. The last day, subjects collected their 24hr urine. Genotoxicity of urinary extracts was assessed by cometand micronucleus assays on a HepG2 cell line. Using competitive enzymatic immunoassay we evaluated the 24hrurinary 8-oxodG excretion. Benzo(a)pyrene [B(a)P] toxicity equivalent factors and inhalation unit risk for B(a)P andbenzene were used to give an estimate of cancer risk levels.Results: Workplace air concentrations of PAHs (e.g. 23.7 [range 2.4-104.6] ng.m-3 for fluoranthene) and VOCs (e.g.19.1±2.9 [standard error] μ.m-3 for benzene) were elevated in sewage compared to office workplaces (P&lt;0.01) andcorresponded to an increased lifetime cancer risk. The urinary extracts of sewage workers showed highergenotoxicity (P&lt;0.001) than office workers. Although not statistically greater than among office workers, the 24hrurinary 8-oxodG mean levels among sewage workers were associated (P=0.04) with years of work in the sewagesystem.Conclusions: The integrated and non-specific urinary biomarkers showed that sewage workers experience exposureto mixtures of genotoxicants in the workplace, and suggest a DNA oxidative stress that might increase with workseniority.</p>en
dc.description.abstract<p>Background: Sewage workers are exposed to multiple chemicals among which many are suspected genotoxicants.Therefore, they might incur DNA damage and oxidative stress. We aimed to explore integrated biomarkers ofexposure and of early effects, respectively, among sewage workers, assessing the overall urine genotoxicity by invitro comet and micronucleus assays and measuring urinary 8-oxo-2'-deoxyguanosine (8-oxodG).Methods: During three consecutive working days, polycyclic aromatic hydrocarbons (PAHs) and volatile organiccompounds (VOCs) were sampled in workplace air of 34 sewage and 30 office workers, as indicators of airborneexposure. The last day, subjects collected their 24hr urine. Genotoxicity of urinary extracts was assessed by cometand micronucleus assays on a HepG2 cell line. Using competitive enzymatic immunoassay we evaluated the 24hrurinary 8-oxodG excretion. Benzo(a)pyrene [B(a)P] toxicity equivalent factors and inhalation unit risk for B(a)P andbenzene were used to give an estimate of cancer risk levels.Results: Workplace air concentrations of PAHs (e.g. 23.7 [range 2.4-104.6] ng.m-3 for fluoranthene) and VOCs (e.g.19.1±2.9 [standard error] μ.m-3 for benzene) were elevated in sewage compared to office workplaces (P&lt;0.01) andcorresponded to an increased lifetime cancer risk. The urinary extracts of sewage workers showed highergenotoxicity (P&lt;0.001) than office workers. Although not statistically greater than among office workers, the 24hrurinary 8-oxodG mean levels among sewage workers were associated (P=0.04) with years of work in the sewagesystem.Conclusions: The integrated and non-specific urinary biomarkers showed that sewage workers experience exposureto mixtures of genotoxicants in the workplace, and suggest a DNA oxidative stress that might increase with workseniority.</p>ar
dc.identifier.urihttps://hdl.handle.net/20.500.11888/9461
dc.titleIntegrated Exposure Assessment of Sewage Workers to Genotoxicants: An Urinary Biomarker Approach and Oxidative Stress Evaluationen
dc.titleIntegrated Exposure Assessment of Sewage Workers to Genotoxicants: An Urinary Biomarker Approach and Oxidative Stress Evaluationar
dc.typeOther
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