Phytochemical Profiling of Arum Palaestinum (Araceae) Leaves by Using HPLCDAD- MS2
Date
2015-04-21
Authors
Abu-Reidah, I.M.
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Abstract
Arum palaestinum is a species of flowering herbaceous perennial
plant belonging to the Araceae family and native to the Mediterranean region.
Arum is an edible plant and is widely used in Traditional Arabic Palestinian Herbal
Medicine (TAPHM). The plant was found amongst the most potent anti-cancerous
plant in Palestine. This besides, its effective action against internal bacterial
infections and poisoning. These properties have been partly assigned to the
phenolics and triterpenes composition. However, only few works have reported
the phytochemical composition of A. palaestinum. In this context, we aimed to
characterize the phytochemical composition of the hydro-methanol extract of A.
palaestinum leaves by means of HPLC-DAD-ESI-MS2. Leaf samples were extracted
with aqueous methanol, centrifuged, followed by supernatant collection,
evaporated, and finally recovered with aqueous methanol. The analysis of the
phytochemicals from A. palaestinum extract was carried out on an Agilent 1200
series LC equipped with an Agilent Zorbax C18 column. Acidic water and
acetonitrile, were used as mobile phases. The HPLC system was coupled to Q-TOFMS,
equipped with an (ESI) source operated in the negative ion mode over the
range from m/z 50-1100. In the present work, more than 160 phytochemical
compounds have been identified in A. palaestinum, highlighting the importance of
this plant as a functional food and as a promising source of bioactive
phytochemicals.