Scientific Conference for Agricultural Research (SCAR2012)

To explore the dogs role in distribution of Human Coetaneous leishmaniasis in Syria, 40 dogs blood samples where chosen randomly in districts of Hama, Idlep, Aleppo, Homs and Lattakia distributed in regions of Hama city, Hurbnefseh, Kufrtanour, Maara, Sheikhbahar, Menan, Hadedah, Houleh and Esawe`eh. DNA extraction was done in graduate studies laboratory in veterinary collage. Polymerase chain reaction PCR test for Leishmania tropica –the main causative of human coetaneous Leishmaniasis- was done and showed positive results in 8 samples(20%) of total cases, the highest prevalence of Leishmania tropica was in Hama 50% followed by (Menan, Hadedah, Kufrtanour- Sheikhbahar) (22.2%, 21.4%, 20%) respectively . The fast strep test (rK-39) specific for L. infantum the causative of human coetaneous Leishmaniasis according to recent studies - on 217 dogs in many districts showed a positive result on 55 dogs (25.3 %) of total cases. 23% of dogs were infected with L. infantum in human coetaneous Leishmaniasis endemic areas, 11% in sporadic human coetaneous Leishmaniasis areas, 28% in human visceral Leishmaniasis areas, 32% in human visceral and coetaneous Leishmaniasis districts . Results reveals a significant variation between dogs infection and owners cultural economic situation, it was (53%, 38%, 9%) in the situations (poor, medium, high) respectively. A primary study in Hama and Aleppo districts on ten peoples infected with Leishmania lesions using (rK-39) fast strep tests –specific for L. infantum – shows positive result in two cases (20%) of total cases