An-Najah National University Faculty of Graduate Studies The Impact of Exclusive Breastfeeding on Infant Morbidity in the First Six Months of Infant s life in Nablus s Refugee Camps By Shaden Hamdi Shakeb Qanadelo Supervised by Dr-Samar Ghazal / Musmar Submitted in Partial Fulfillments of the Requirements for the Degree of Master in Public Health, Faculty of Graduate Studies, at An- Najah National University, Nablus, Palestine. 2010 iii Dedication To my parents, to every one deprived from education, and to those who know the value of learning and knowledge. iv Acknowledgement ALLAH, Subhanahu wa ta'ala said in holy Quraan:- And Allah's grace on you is very great. (Surah An-Nisaa, 113) Firstly I must thank God for his graces and blessing on me to complete this study, which helped me to overcome all obstacles and hard times I met throughout my study years. I would like to extend my sincere gratitude to my thesis supervisor Dr-Samar Ghazal\ Musmar, MD; without her this research would never have been achieved. I would like to thank her deeply for her invaluable expert scientific guidance, support, and advises My sincere thanks to Dr-Motasim Al-Masri, and Dr- Suhail Salha for their scientific advises and continuous support. From bottom of my heart I would like to thank my parents who taught me the value of learning, especial thanks for my mother who's been always supporting, advising, and ensuring me that by believing, hope, and hard work all hard targets would be possible. v : The Impact of Exclusive Breastfeeding on Infant Morbidity in the First Six Months of Infant s life in Nablus s Refugee Camps . Declaration The work provided in this thesis, unless otherwise referenced, is the researcher s own work, and has not been submitted elsewhere for any other degree or qualification. : Student's name: : Signature: : Date: vi List of Acronyms Abbreviation Explanation AAFP The American Academy of Family Physicians. AAP The American Academy of Pediatrics. ACOG The American College of Obstetricians and Gynecologist. ALRI Acute Lower Respiratory Infection AOM Acute Otitis Media ARI Acute Respiratory Infections. BF Breastfeeding. EBF Exclusive breastfeeding. EFF Exclusive Formula milk Feeding. IgA Immunoglobulin A. IgD Immunoglobulin D. IgE Immunoglobulin E. IgG Immunoglobulin G. IgM Immunoglobulin M. LRTI Lower Respiratory Tract Infection. MCH Maternal and Child Health OM Otitis Media. Adj-OR Adjusted Odds Ratio PBF Partial Breastfeeding. PCBS Palestinian Central Bureau of Statistics. PMOH Palestinian Ministry of Health. PSC Palestinian Satellite Channel ROM Recurrent Otitis Media. S-IgA Secretory Immunoglobulin A. SPSS Statistical Package for Social Sciences. Type I DM Type I Diabetes Mellitus. UNICEF The United Nations Children s Fund. UNRWA United Nations and Works Agency For Palestine Refugees in the Near East. URTI Upper Respiratory Tract Infection. USA The United States of America. UTI Urinary Tract Infection. WHO World Health Organization. vii Table of Contents. No. Subject Page Dedication iii Acknowledgement iv Declaration v List of Acronyms vi Table of contents vii List of tables x List of Appendix xi Abstract xii Chapter One: Introduction 1 1.1 Study background 2 1.2 Types and importance of breastfeeding 3 1.3 Types, composition, and benefits of Breast milk 5 1.3.1 Biological and chemical composition of breast milk 6 1.3.2 Breast milk role in fighting infection 8 1.3.3 Other benefits of breast milk 10 1.4 Formula feeding 10 1.5 The problem of the study 11 1.6 Significance of the study 13 1.7 Goal and objectives of study 14 1.8 Study questions 15 1.9 Important definitions for this study 17 Summary 18 Chapter Two: Literature review 19 2.1 Relationship of breastfeeding and infections studies at international level 20 2.2 Regional studies 23 2.3 Breastfeeding studies at national level 25 Summary 26 Chapter Three: Methodology and procedure 27 3.1 Study population 28 3.2 Study design and sampling method 30 3.3 Sample of the study 30 3.3.1 Inclusion criteria 31 3.3.2 Exclusion criteria 32 3.4 Operational definition of the study variables 33 3.5 Instrument of data collection 37 3.5.1 Questionnair 37 3.5.2 Reliability and Validity of the tool 38 3.5.3 Data collection 39 viii No. Subject Page 3.6 Potential confounders 39 3.7 Data analysis 39 3.8 Ethical issues 41 3.9 Study limitations 41 Summary 42 Chapter four: Results 43 4.1 Sample distribution according to mother's and infant's sociodemo-graphic profile 44 4.2 Sample distribution according to infant s history of morbidity in the first six months 46 4.3 Sample distribution according to type of infant feeding in the first six months of life 48 4.4 Results related to study hypotheses 48 4.4.1 Results related to the specific hypotheses; relationship between selected mother s and infant s sociodemographic profiles and type of feeding in the first six months of life in Nablus refugee camps 48 4.4.2 Results related to the hypotheses of morbidity 54 4.4.2.1 Results related to the first specific hypothesis; relationship between type of feeding and frequency of disease visits in the first six months of life in Nablus refugee camps 54 4.4.2.2 Results related to the main hypothesis; relationship between type of infant feeding and morbidity in the first six months of infant s life in Nablus refugee camps 55 4.4.3 Relationship between type of infant feeding and morbidity after adjustment for mother and infant confounding factors 59 Summary 61 Chapter five: Discussion 62 5.1 Discussion related to sample distribution according to mother s and infant s sociodemographic profile 63 5.2 Discussion related to sample distribution according to infant history of morbidity in the first six months 65 5.3 Discussion related to sample distribution according to type of infant feeding in the first six months of life 66 5.4 Discussion related to study hypotheses 67 5.4.1 Discussion related to the specific hypotheses; relationship between selected mother s and infant s sociodemographic profiles and type of feeding in the first six months of life in Nablus refugee camps 67 ix No. Subject Page 5.4.2 Discussion related to the hypotheses of morbidity 72 5.4.2.1 Discussion related to the first specific hypothesis; relationship between type of feeding and frequency of disease visits in the first six months of life in Nablus refugee camps 72 5.4.2.2 Discussion related to the main hypothesis before and after adjustment for confounding factors; relationship between type of infant feeding and morbidity in the first six months of infant s life in Nablus refugee camps 73 Summary 81 Chapter Six: Conclusions and Recommendations 82 6.1 Conclusions 83 6.2 Recommendations 84 Summary 86 References 87 Appendix 100 x List of Tables No. Table Page Table (1) Sample distribution according to the place of resident 31 Table (2) The sociodemographic profile of the study sample for both mothers and infants 45 Table (3) Sample distribution according to infant s history of morbidity in the first six months of life 46 Table (4) Sample distribution according to infant s morbidity with different diseases visits in the first six months of life 47 Table (5) Type of feeding in the first six month of life in the study sample 48 Table (6) Relationship between different mother's and infant's sociodemographic variables and type of feeding in the first six months of life 50 Table (7) Relationship between type of feeding in the first six months of life and infant s frequency of disease visits in the same period of age 55 Table (8) Relationship between type of infant feeding in the first six months of life and morbidity according to different diseases in the same age 56 Table (9) Adjusted Odds ratio, Confidence Intervals and Significance levels for diseases affected by type of feeding 60 xi List of Appendix No. Appendix Page Appendix (1) Questionnaire 101 Appendix (2) Commitments for UNRWA and agreement from them to conduct the study 104 Appendix (3) Child health record girls 0-3 years 105 xii The Impact of Exclusive Breastfeeding on Infant Morbidity in the First Six Months of Infant s life in Nablus s Refugee Camps By Shaden Hamdi Shakeb Qanadelo Supervised by Dr: Samar Ghazal / Musmar Abstract Throughout infancy it is important to receive the appropriate and the adequate nutrition, to ensure the utmost extreme biological growth and mental development for the child. World wide, many studies on infants provided evidences that breastfeeding is the ideal method; it can decrease the incidence and severity of many infectious conditions, such studies in our country are lacking. This study aimed to explore the effects of the different infant feeding patterns on infant s morbidity in the first six months of age in Nablus refugee camps (Balata, Askar, and Ein Beit el Ma). The research also aimed to explore the relationship between selected mother s and infant s sociodemographic factors, and the choice for the infant's patterns of feeding during the first six months of life in Nablus refugee camps. The study was cross-sectional retrospective analytical survey, included 690 files of infant\mother pair s, born in 2007, reside in Nablus refugee camps, and receive health care in one of three UNRWA's clinics in Nablus refugee camps. Secondary data obtained from infants clinical files, then analyzed by SPSS program, version 11; frequencies and percentages, Pearson Chi-square, and multiple logistic regression methods were used. xiii The study results indicated; that percentage of Excusive Breastfeeding (EBF) for the first six months of life was 70%, whereas Partial Breastfeeding (PBF) was 16%, and Exclusive Formula Feeding (EFF) was 14%. Which reflected the good efforts regarding promoting exclusive breastfeeding in the UNRWA's clinics. Mother and infant sociodemographic factors (mothers aged 22-28 years olds, mother aged 15-22 years olds at marriage, mother aged 22-28 years old at delivery, those with high parity >4, mothers with basic and secondary level of education, not educated fathers, vaginal delivery, and female infants) were positively related to EBF significantly at 0.05.. On the other hand, EBF in the first six months of infant s life was negatively related to mothers aged > 36 years olds, mother aged > 29 years olds at marriage, mother aged > 36 years old at delivery, and those with low parity 1-2, high educated mothers and fathers, cesarean delivery, and male infants. This study confirmed that exclusive breastfeeding during the first six months of life protects against, lower respiratory tract infection, otitis media, gastroenteritis, diarrhea, wheezing, and allergies. The effect of protection continued to be statistically significant (except for diarrhea) even after adjusting for mother and infant sociodemographic factors. In conclusion, this study confirms that type of infant feeding in the first six months of infant's life is affected by mothers and infant's xiv sociodemographic variables. And that exclusive breastfeeding in the first six months of life is important element in preventing and decreasing many diseases during the same period of age. The study results recommend that health agencies must give more concern for breastfeeding in general and exclusive breastfeeding in particular, especially during planning for fighting against infectious diseases. Further studies regarding this subject must be conducted within the Palestinian health agencies. 1 Chapter one Introduction 2 Chapter one Introduction Breastfeeding has received significant attention in the past twenty years. Exclusive breastfeeding and its impact on infant's health has been a field of medical and public health research world wide. UNRWA's services at Palestinian Refugee camps are keeping excellent health records for infants and their mothers suitable to this type of study. In this chapter, the study background, the research problem, significance of the study, goal and objectives, study questions, hypotheses, and important definitions for what will be presented. 1.1.Study background Having the appropriate and the adequate nutrition during infancy, and early childhood are fundamentals to the development of each child s full human potential 1. It is well recognized that the first two years of age is a critical window for the promotion of optimal growth, health, and behavioral development 1. This is due to the fact that this age is the peak for growth faltering, deficiencies of certain micronutrients, and common childhood illnesses such as diarrhea 1. Numerous factors affect the mother's choice to breastfeed, including socioeconomic status, cultural beliefs, level of social support, level of education, maternal work demands, range of care interventions provided 3 during pregnancy, childbirth and the early postpartum period (medical advice), family pressures and commercial advertising 2,3. Biological factors including infant size, sex, development, interest/desire, growth rate, appetite, physical activity, and maternal lactational capacity may influence the decision about the type of feeding for infant, supplementation, and also determine the need and timing of complementary feeding 2,3. 1.2.Types and importance of breastfeeding Breastfeeding (BF) is the ideal method of providing young infants with the optimum form of nutrients they require for healthy growth and development 4. By receiving accurate information, and the needed support of their families and the health care system; virtually all mothers can breastfeed 4. The World Health Assembly, endorsed a resolution in May 2001 advocating the need to encourage Exclusive Breast Feeding (EBF) up to the age 6 months taking into account the findings of the World Health Organization (WHO) expert consultation on optimal duration of EBF; WHO s public health and infant feeding recommendations is that infants should be exclusively breastfed during the first six months of life and that they should continue to receive breast milk throughout the remainder of the first year and during the second year of life or beyond, and unrestricted exclusive breastfeeding results in ample milk production. 5, 6,7, 8,9. To enable mothers to establish and sustain EBF for 6 months, WHO and the United Nations Children s Fund (UNICEF) recommend 8: 4 Within the first hour of life, mother can initiate breastfeeding. Exclusive breastfeeding. Breastfeeding on demand according to the child need, day and night. No use of bottles, teats or pacifiers. According to WHO report in the year 2002, and the American Academy of Pediatrics (AAP) in the year 2005, the definition of Exclusive Breastfeeding (EBF) is that the infant only receives breastmilk, and no water, other fluids (juice, non-human milk), or foods should be administered 7, 8, 10. EBF definition also comprises that the infant receives no pacifiers/dummies or artificial teats, at least 8-12 breastfeeding times in 24 hours, including the night ones especially for newborn babies 11. It must be clear that any limitations placed on the number and /or duration of breastfeeding will interfere with the meaning of EBF 11. When infant receives breast milk plus infant formula, this pattern of feeding is called mixed feeding 10. Both the American Dietetic Association and the AAP (2005) recommended EBF for the infant's first 6 months of life, and to continue breastfeeding in conjunction with other foods for the first year 2, 10. Breastfeeding has been promoted and\ or recommended also by the United States Department of Health and Human Services, the American 5 Public Health Association, the American Academy of Family Physicians (AAFP), the American College of Obstetricians and Gynecologists (ACOG), and numerous other institutions and organizations 12, 13. Islam as a religion strongly promotes breastfeeding. In the following ayah (verse) from the Holly Quran, ALLAH, Subhanahu WA ta'ala recommends the mother to suckle her offspring for 2 years if possible 14. The mothers shall suckle their offspring for two whole years, (that is) for those (parents) who desire to complete the term of suckling (Al- Baqarah: 233) At national level, the Palestinian Ministry of Health (PMOH) coordinated with Palestine television and Palestinian Satellite Channel (PSC) through production of many programs to promote awareness for breastfeeding 15. PMOH also plays a role through implementation of many programs on the importance of breastfeeding targeting women inside women unions, institutions, and health clinics, in addition to offering training courses in breastfeeding for participants from health organizations, and distribution of booklets and posters to targeted groups 16. 1.3.Types, composition, and benefits of breast milk According to WHO In almost all situations, breastfeeding remains the simplest, healthiest and the least expensive, natural first food for babies 7, 8. Human milk provides easily tolerated, digestible, readily available at any time and everywhere (requires no preparation), sterile 6 formula, at proper temperature, and with no artificial colorings, flavorings, or preservatives 2, 11. Sometimes breastmilk called the white blood, since it is considered similar to the placental blood of intrauterine life, in addition it is similar to unstructured living tissue like blood, plus its capability of transporting nutrients, affecting biochemical systems, enhancing immunity, and destroying pathogens 17. Like all other animal milks; human breastmilk is species-specific, its own ideal composition makes it different from that of other mammals types regarding ingredients and their concentrations 17, 18, 19. This composition is not constant and varies with stage of lactation, breastfeeding pattern, season, and parity, and differs also among individuals (mother s milk is especially suited for her own baby) and communities, and are adapted to gestational age 11,18. Mature breastmilk changes from month to month, day to day, feed to feed which makes it the superior milk that meets each particular baby s needs, plus that human breastmilk adapted throughout human existence to meet nutritional and anti-infective requirements of the human infant to ensure optimal growth, development, and survival11, 17, 18. 1.3.1. Biological and chemical composition of breast milk The chemical composition of breast milk changes dramatically over time in the postpartum period. The stages of lactation correspond roughly 7 to the postpartum time: colostrum (0 5 days), transitional milk (6 14 days), and mature milk (15 30 days) 2,20 As a complex fluid; breastmilk contains all the energy and nutrients needed by the newborn baby to promote infant s health, growth, and development during the first six months of life 7,8,18. It continues to provide up to half or more of a child s nutritional needs during the second half of the first year, and up to one-third during the second year of life 7,8 . Nutrients include metabolic fuel (fat, protein, and carbohydrates), water, and the raw materials essential for tissue growth and development, such as fatty acids, amino acids, adequate minerals, vitamins, and trace elements 18. It is important to know that the presence of some proteins in human milk are not for nutritional purpose to the infant; it serves immunological role instead 17. For example breastmilk as a white blood contains (leukocytes, immunoglobulins, and enzymes) in abundance, and the nonantibody factors (Lactoferrin, the bifidus factors, and oligosaccharides) which offer the newborn protection against disease; this benefit in addition to other benefits has been recognized for hundreds of years and makes it a peerless substance for feeding the human infant 17. As a white blood there are two types of leukocytes in human milk; phagocytes (90%) and lymphocytes (10%) and their concentrations vary with the duration of lactation; where after birth these cells are higher than at any other time 17. 8 Macrophages are the dominant phagocytes 17. They engulf and absorb pathogens, release Immunoglobulin A (IgA), and produce lactoferrin and lysozyme also 17. Neutrophils are other phagocytic leukocytes in breast milk and the first to arrive to the inflamed site 17. Lymphocytes in breast milk include T-cells (83%) and B-cells provide helpful immunity important in the destruction of viruses 17, 21. 1.3.2. Breast milk's role in fighting infections Antibodies (immunoglobulins) are proteins produced by plasma cells in response to an immunogen 17. All types of immunoglobulins (IgG, IgA, IgM, IgE, and IgD) are found in human milk; however, both IgA and IgE, play a critical role in biological specificity of human milk on the recipient infant17, 22. Secretory IgA (S-IgA) a compound that is the primary disease fighter in the human immune system, reflects mother's exposure to mucosal infection, attaches to the lining of the nose, mouth, and throat, and fights the attachment of specific infecting agents 17,18,19,22. S-IgA is a couple of two IgA molecules, so-called secretory component seems to shield the antibody molecules from being degraded by the gastric acid and digestive enzymes in the stomach and intestines, this characteristic helps it to reach the intestine in undamaged condition, where it acts on pathogenic organisms and inhibit their multiplication 19,22,23 . The immunization process begins at birth by passive immunity, because mother s milk has antibodies to protect babies against diseases to 9 which they might exposed to 2,11. Infant s own immune system may also produce S-IgA, but under the age of two years infant has immature immune response that is sometimes incapable of preventing disease 19. The consumption of the mother's S-IgA not only provides resistance to disease, but it also stimulates the production of additional S-IgA in the infant 19. As a result; breastfed children exhibit greater resistance to infectious diseases and stronger immune systems than their formula fed peers 19. Breastmilk also supplies smaller amount of IgM and IgG 24. Lysozyme; a major component of human milk enzymes (a potent digestive ingredient), has both bacteriocidal and anti-inflammatory action, and is much more abundant in human milk than in bovine type 17. Nonantibody factors in human milk comprise an elegant and intricate system that protects the infant against bacterial infection; the most important ones of these factors are Lcatoferrin, the bifidus factors, and oligosaccharides 17. Lactoferrin; is a potent iron-binding protein which absorbs enteric iron upon reaching intestine, leading to reduction of the amount of free iron below the level necessary for the growth of iron-dependent bacteria 24,17, 22. The intestinal flora of breastfed infants is dominated by gram- positive helpful bacteria called Lactobacillus bifidus; with a level in breast- fed infants that is typically ten times greater than that of formula-fed infants17, 22. The bifidus factor in human milk promotes growth of these beneficial bacteria17. The buffering capacities of milk (bifidus factor) 10 together with the lactobacillus, contribute to the low pH (5-6) of stool 17, 24. This acid environment will discourage the replication of many pathogens17. Oligosaccharides (carbohydrates composed of a few monosaccharides) in human milk help to block antigens from attaching to the epithelium of the gastrointestinal tract17. 1.3.3. Other benefits of breastfeeding Breastmilk in general provides many benefits for the baby's health; for example, it promotes optimal mother-infant bonding, and if the breastfeeding is exclusive will reduce spending on infant formulas and the benefits will increase to its highest; healthier mother and infant, reduce the need for the different types of medical services 2,11,19. The choice to breastfeed has a potential for decreasing annual health costs in The United States of America (USA) by $3.6 billion and decreasing parental employee absenteeism, the environmental burden for disposal of formula cans and bottles, and energy demands for production and transport of formula 10. So, in general breastfeeding is economic element for society 11. 1.4.Formula feeding Infant formula is an artificial substitute for human breast milk, designed for infant consumption 25.Today, most infant formulas are based on either cow milk or soy milk, and some other types for infants with special dietary needs, are highly modified and may contain neither cow milk nor soy 25. 11 Breastmilk substitutes increase the risk of contamination either during manufacturing or home preparation; for example, water used for washing bottle or mixing infant formula may be contaminated. Some families may dilute it to make it last longer or fall in errors during mixing11. 1.5.The problem of the study Worldwide, diarrhea and respiratory infections are major causes of death of children, especially young ones 26. Acute gastroenteritis is one of the leading causes of illness and death in infants and children throughout the world, especially in developing countries; an estimated 2.5 million gastroenteritis deaths occur each year in children less than 5 years of age26,27. In Palestine, diarrhea is one of the main causes of outpatient visits and hospitalizations28. In the Palestinian Territory, deaths related to respiratory diseases are the second cause of infant death (21.8%) for the year 2005 29. Breastfeeding benefits were investigated by many studies which concluded that breastfed children exhibit greater resistance to infectious diseases and stronger immune system, with a lower rate of chronic diseases when compared with those fed by formula 19. Moreover, according to WHO report (2002); EBF during the first months of life is considered as a significant element that reduces infant morbidity and mortality, and aids in a quicker recovery during illness, especially those related to diarrheal disease and acute respiratory infections 7. 12 The evidence of this protection was studied worldwide, and demonstrated that these effects are even more important in resource-poor, mal-nourished, and heavily populated societies 8, 17. Consequently the very cramped, poor sanitation, and overcrowd Palestine refugee camps in general are considered the ideal site for such an important study lacking in Palestine. In Nablus there are three refugee camps suitable to conduct this study; Balata, Askar, and Ein Beit el Ma. However, no previous studies were found to detect for the relationship between type of feeding in the first six months of infant life, and infant morbidity regarding different and important diseases in the same period of infant's age in Palestine at all. Examples are; upper respiratory tract infection, lower respiratory tract infection, otitis media, asthma, wheezing, gastroenteritis, diarrhea, urinary tract infections, and allergies. In addition, there is a lacking in the studies that investigated in details the relationship between mother s and infant's sociodemographic factors and choice of infant s patterns of feeding during the first six months of life in Palestine. Examples are; mother s age, mother s age at marriage, mother s age at baby s birth, mother s level of education, mode of delivery, parity, and infant s gender. This study also tried to search for the role of father in affecting the mother's decision of type of feeding their infants in the first six months of life, especially father's level of education. 13 Throughout this research, the researcher wants to investigate for the importance of exclusive breastfeeding as a simple and cheap method that fight the infectious diseases which increase the economic and medical burdens, especially in a developing society like Palestine refugee camps. And the researcher will try to detect the sociodemographic factors that negatively affect the exclusive breastfeeding to focus the promoting efforts on mothers with low tendency to exclusively breastfeed. 1.6. Significance of the study This research is considered as the first study in Palestine refugee camps, particularly Nablus ones that aimed to investigate the relationship between type of feeding in the first six months of infant life, and infant morbidity in the same period of infant's age. It focuses on different diseases that affect many infants inspite it could be preventable by very simple and cheap methods. Examples are; upper respiratory tract infection, lower respiratory tract infection, otitis media, asthma, wheezing, gastroenteritis, diarrhea, urinary tract infections, and allergies. This research also aimed to find any relationship between selected mother's and infant's sociodemographic variables and the mother's choice of infant's feeding in the first six months of infant life. Many previous studies improved the presence and in some times the absence of a possible statistical relationship, between mother's and infant's 14 sociodemographic variables and the choice for the type of infant's feeding in the first six months of life. Examples are mother s age, mother s age at marriage, mother s age at baby s birth, mother s level of education, mode of delivery, parity, and infant s gender. In addition, some studies investigated for the role of the father in affecting the mother choice on their infant feeding especially regarding a variable like father's level of education. It is expected that this study will play an important role in highlighting the importance of exclusive breastfeeding in the first six months of infant's life on infant morbidity. It also is expected to highlight factors affecting breastfeeding in Palestinian refugee camps' societies. 1.7. Goal and objectives of study Study goal This research aims to explore the effect of the different types of feeding (exclusive breastfeeding, partial breastfeeding, and exclusive formula feeding) on infant morbidity in the first six months of age in Nablus refugee camps. Specific objectives In order to reach the main study goal, the following objectives are set to be measured. 15 1. To explore the relationship between type of feeding and frequency of disease visits in the first six months of life in Nablus refugee camps (examples are; upper respiratory tract infection, lower respiratory tract infection, otitis media, asthma, wheezing, gastroenteritis, diarrhea, urinary tract infections, and allergies). 2. To explore possible relationship between selected mother s demographic factors and choice of infant s patterns of feeding during the first six months of life in Nablus refugee camps (examples are mother s age, mother s age at marriage, mother s age at baby s birth, mother s level of education, mode of delivery, and parity). 3. To explore possible relationship between infant s gender and choice of infant s pattern of feeding during the first six months of life in Nablus refugee camps. 4. To identify possible relationship between father's level of education and type of infant feeding during the first six months of life in Nablus refugee camps. 1.8. Hypotheses Main hypothesis There is no significant relationship, at the significance level p 0.05, between type of infant feeding and morbidity in the first six months of infant s life in Nablus refugee camps. 16 Specific hypotheses 1. There is no significant relationship, at the significance level p 0.05, between type of feeding and frequency of disease visits in the first six months of life in Nablus refugee camps (examples are; upper respiratory tract infection, lower respiratory tract infection, otitis media, asthma, wheezing, gastroenteritis, diarrhea, urinary tract infections, and allergies). 2. There is no significant relationship, at the significance level p 0.05, between mother s age and type of feeding in the first six months of life in Nablus refugee camps. 3. There is no significant relationship, at the significance level p 0.05, between mother s age at marriage and type of feeding in the first six months of life in Nablus refugee camps. 4. There is no significant relationship, at the significance level p 0.05, between mother s age at the time of infant s birth and type of feeding in the first six months of life in Nablus refugee camps in Nablus refugee camps. 5. There is no significant relationship, at the significance level p 0.05, between parity and type of feeding in the first six months of life. 6. There is no significant relationship, at the significance level p 0.05, between mother s level of education and type of feeding in the first six months of life in Nablus refugee camps. 17 7. There is no significant relationship, at the significance level p 0.05, between father s level of education and type of feeding in the first six months of life in Nablus refugee camps. 8. There is no significant relationship, at the significance level p 0.05, between mode of delivery for the infant who participated in the current study and type of feeding in the first six months of life in Nablus refugee camps. 9. There is no significant relationship, at the significance level p 0.05, between infant s gender and type of feeding in the first six months of life in Nablus refugee camps. 1.9. Important definitions for this study: United Nations Relief and Works Agency for Palestine Refugees in the Near East (UNRWA) is a relief and human development agency, providing aids to Palestine refugees living in Jordan, Lebanon and Syria, as well as in the West Bank and the Gaza Strip 30. Palestinian refugees or Palestine refugees are the people and their descendants, predominantly Palestinian Arabic-speakers, who fled or were expelled from their homes during and after the 1948 Palestine War 31. Exclusively breastfed infants (EBF); infants who receive only Breastmilk and no water or other fluids (juice, non-human milk) or foods7,8,10. 18 Partially breastfed (PBF); Combination of breast feeding with formula milk 10. Infant formula; is an artificial substitute for human breast milk, designed for infant consumption 25. According to this study, feeding the infant by formulas only during the first six months of life is defined as Exclusive Formula Fed (EFF). According to this study, definition of disease visit was actual clinic's visit or request for medical prescription for any treatment in other places. Odds ratio; is the ratio of the probability of the two possible states of a binary variable in two groups of subject 32. Summary Breastfeeding is considered and recommended as the ideal way of infant feeding by many health agencies and Islam. Exclusive breastfeeding in the first six months of life is found to be an important element in reducing health and economic burdens of infectious diseases especially those related to diarrheal and respiratory infections. Consequently, breastfeeding in general and exclusive type in particular deserves to be studied and focused on especially when we plan for development of child health especially in our developing country (Palestine). 19 Chapter Two Literature review 20 Chapter Two Literature review This chapter, briefly presents important scholarly work which investigated breastfeeding. Benefits and role in decreasing morbidity, important statistics of breastfeeding at international, and regional, and national levels are presented within this chapter. 2.1. Relationship of breastfeeding and infections studies at international level Exclusive breastfeeding for 6 months is not a common practice in developed countries, and is still rarer in the developing ones 8. In its Healthy People 2010 statement; the United States has established federal goals for breastfeeding, including that for initiation in the early postpartum period, the percentage is 75%, continuing to 6 months postpartum is 50%, and of all women breastfeeding 25% which must continue to 1 year, exclusive breastfeeding for 3 months is 60% and for 6 months is 25% 33. According to UNICEF statistics about EBF in different regions in the world for the years 1995 to 2000, and the year 2006, less than half were EBF < 4 months in most countries 34, 35. Several studies in different western countries explored protective effect of breastfeeding against infections in the first six months of infant s life. Howie et al designed a prospective observational study in Dundee 21 (Scotland) published in 1990, and concluded that breast feeding when compared with bottle-feeding, confers protection against gastrointestinal illness; that persists beyond the period of breast feeding itself 36. Quigly et al (1990) conducted a case control study in England and found that: after adjustment for confounders; breast feeding protects against diarrheal disease in infants aged 6 months 37. Chantry et al study (1988-1994) documented the importance of full breastfeeding for 6 months (Full\BF 6) in providing greater protection against respiratory tract infection than does full breastfeeding for >4 but <6 months in the USA 38. In addition, infants exclusively breastfed for one to three months Full\BF1-3 group had the first episode of OM before 1 year of age and Recurrent Otitis Media (ROM) more than the Full\BF 6 group 38. Levine et al carried out a case-control study in North America (1995- 1996) and found that; among infants 2-11 months old, current breastfeeding was associated with a decreased likelihood of invasive pneumococcal disease (Streptococcus pneumoniae) 39. Galton Bachrach et al (2003) conducted a meta-analysis of 33 studies on infants in developed nations to investigate the relation between breastfeeding and the risk of hospitalization for lower respiratory tract disease (LRTD) 40. They concluded that among generally healthy infants, those who were not breastfed had developed severe respiratory tract illnesses resulting in hospitalization three times more compared to those who were exclusively breastfed for 4 months 40. 22 Similar findings in New Mexico, USA was illustrated by Cushing et al (1988-1992) who found that full breastfeeding was associated with a reduction in lower respiratory illness, and that breastfeeding reduces the severity of infant respiratory illnesses during the first 6 months of life 41. Several studies concentrated on the duration of breastfeeding and its relationship with infection. Sassen et al study carried out (1987-1988) in Leiden (Holland) revealed that the risk of Acute Otitis Media (AOM) depends on the number of months an infant is breast-fed and the number of months that pass after breast-feeding is discontinued 42. Duncan et al study in Tucson, USA (1993) suggests that EBF for 4 or more months protects infants from OM and ROM 43. A study in Mexico City carried out by L pez-Alarc n et al (1997) showed that the likelihood of having a larger percentage of days ill was higher for the formula-fed than for the fully breast-fed infants during the entire follow-up. It concluded that human milk has a protective effect for Acute Respiratory Infection (ARI), as it does for diarrhea 44. Two Brazilian studies explored breastfeeding and infection relationship. César et al study (1993) found that breastfeeding protects young Brazilian children against pneumonia, especially in the first months of life (under 3 months old) 45. Romieu et al study (2000) confirmed that the low prevalence of asthma and wheezing observed among Brazilian school children (7-14) years of age may be partly related to the high level of breastfeeding 46. 23 In the Philippines, Yoon et al (1988-1991) found that children who were not breastfed during the first 6 months of life, compared with those who were breastfed, were more likely to die of diarrhea 47. He also found a six times increase in mortality associated with both acute lower respiratory infection (ALRI) and diarrhea combined by not breastfeeding in the first 6 months of life. Unfortunately, the data also suggested that after age 6 months, the protective effects of breastfeeding dropped dramatically 47. 2.2. Regional studies The effect of breastfeeding on infant mortality and morbidity has been studied in some regional countries. In Bangladesh, Arifeen et al cohort study (1993- 1995) showed that infants who were either partially or not breastfed had a higher risk of post neonatal (29 days-11 months) death than infants who were breastfed exclusively for the first 4 months of life 48. Another study in Bangladesh carried out by Mihrshahi et al (2003), showed that children aged 0-3 month(s) who are EBF, were less likely to have suffered from diarrhea and ARI than infants who were not EBF 49. In Saudi Arabia, a retrospective study carried out by Abdulmoneim I et al (2001) found that during the first 2 years of life; attack rates of all respiratory tract infections was significantly related to duration of lactation50. 24 Another study in Egypt (1995-1997) carried out by Clements et al found that, relative to infants who were not breastfed, those who were breastfed exclusively, or partially breastfed had a 33% and 28% lower rate of diarrhea respectively 51. Some of studies in the region explored effect of sociodemographic factors on breastfeeding pattern and choices. For example, Khassawneh et al study (2003) in Irbid, Jordan, found that the type of feeding was independent of mother's age, father's education level, child's gender 52. Women with higher education (more than high school) were less likely to breastfeed, similarly employed mothers, mothers delivered by caesarian, and those with lower number of children (<3) were less likely to fully breastfeed 52. The study concluded that a high proportion of Jordanian women did breastfeed for more than one year 52. In Israel, a study conducted by Ever-Hadani et al on Jewish women (1974-1976) in the Jerusalem district found that women aged 24 years or younger and those aged 40 years or more were most likely to choose to breast feed. Birth order, maternal education, social class, age at marriage, and work outside the home did not significantly influence the decision to begin breast feeding 53. Delivering by caesarean section was significantly associated with a tendency to formula feed 53. Primipara and grandmultipara (parity > 4) were significantly more likely to breastfeed for three months or more. Mothers with the fewest years of schooling on the one hand, and those with the highest level education on the other, were most likely to breast feed for extended periods 53. 25 2.3. Breastfeeding studies at national level. Although there are very few studies done on the breastfeeding subject in Palestine, a study carried out in Gaza Strip (2007) found that the level of mother's knowledge in regard to breastfeeding doesn t guarantee the full implementation of breastfeeding, therefore; a need for further training to health care providers on the infant feeding strategies and more ministerial commitments toward the implementation of BF strategies is needed 54. According to the Demographic and Health survey conducted in Palestinian Territory in the year 2004, one noticed that the percentage of breastfed children who were born 3 years before the study was 95.6% 55. Also in the report of Palestinian family health survey for the year 2006, which was published by the Palestinian Central Bureau of Statistics (PCBS) in the year 2007, the results showed that breastfeeding practice is common in the Palestinian Territory, since 97.5% of children under five years of age were breastfed 55. It is good to know that the variation in breastfeeding across regions and districts was minimal, and the average duration of breastfeeding was 10.9 months in the year 2004, while in the year 2006 results showed that the average duration of breastfeeding increased to 12 months 29,55. But one will be surprised when he knows that inspite of the high breastfeeding percentage in the year 2006, the percentage of the exclusive breastfeeding the same year in Palestinian Territory was only 24.8% 55. 26 The UNRWA study (2001) that assessed the prevalence and duration of EBF among Palestinian refugee infants utilizing UNRWA services in the five Fields of the Agency s area of operation (Jordan, Lebanon, Syrian Arab Republic, Gaza Strip, and the West Bank) found that the prevalence rates of EBF up to 4 months were (24.0%, 30.2%, 40.3%, 33.3%, 34.5%) respectively, with average 32.7% 5. And the mean duration of EBF agency- wide based on data collected at the date of the interview was 2.7 months with no significant variations between Fields 5. Summary Most of international and regional studies concentrated on importance of breastfeeding in general and exclusive type in particular. At the national level all of the studies concentrated on prevalence and sociodemographic characteristics, but did not explore a possible relationship between breastfeeding and morbidity in children, especially infectious diseases in infancy. The researcher s literature review showed no previous published study at the national level directed towards the impact of exclusive breastfeeding on infant morbidity in the first six months of life in Nablus refugee camps. It is expected that such a study is going to lead to important results in this subject. 27 Chapter Three Methodology 28 Chapter Three Methodology The main aim of this study is to explore the immediate effect of the different types of feeding (exclusive breastfeeding, partial breastfeeding, and exclusive formula feeding) on infant morbidity in the first six months of age in Nablus refugee camps In this chapter the research design and steps utilized to reach this important goal include; study population, study design and sampling method, operational definition of the study variables, instrument of data collection, potential confounders, data analysis, ethical issues, and study limitations. 3.1.Study population The study population was chosen to include infants born in 2007, and registered to get their health care at the maternal and child health departments, in all the UNRWA's clinics included at Nablus area. There are 27 refugee camps in the occupied Palestinian territory (19 in West Bank) 56. Approximately one third of Palestinian refugees still live in refugee camps; making up 25.4% of all refugees in the West Bank 56. Population density (inhabitants/km2) is high throughout the occupied Palestinian territory, it increased from 481 in 1997 to 626 in 2007 (416 in the West Bank) 57. According to WHO agenda (2008), the occupied Palestinian territory has a young population 57. The proportion of 29 individuals below 15 years of age is slightly higher among refugees (45.8%) than non-refugees (45.3%) 57. The broad unemployment rate in West Bank refugees rose from (24.5%) in 2007 to (25.3%) in 2008 57. Nablus is a big city located in the north of West Bank, Palestine with (320,830) population according to the PCBS (2007) 55. In Nablus there are three refugee camps, Balata camp (in the eastern side of Nablus city) is one of the most densely populated locations on Earth since 30,000 people in its concrete block houses on less than two square kilometers in size 58. In Askar (located in eastern side of Nablus) the camps' registered population is 14,629 live on 299 dunums of land 59, and Ein Beit el Ma camp (located in western part of Nablus) had a population of approximately 5,036 inhabitants on 45 dunums in mid-year 2006 60. Since 1948, UNRWA established 3 clinics in Nablus, providing health and preventive care in the main 3 refugee camps (Balata, Askar, and Ein Beit el Ma) in Nablus area. According to the three clinic records for infants born in the year 2007, and registered at MCH in each one of these clinics; it was found that there are 1738 active files for infants and their mothers in the three refugee camps clinics (756 infants at Balata, 560 at Askar, and 422 at Ein Beit el Ma clinic). 30 3.2.Study design and sampling method This study is a cross-sectional retrospective analytical survey. Only those who met inclusion criteria were included in the study. A total of (690) infant\ mother files from the 3 clinics at refugee camps met the criteria to be included in the study, while 1048 infants were excluded. The time needed for collecting data was 2 months from November 2008 till January 2009. The clinical filling system used by UNRWA contains enough information related to the study concepts in addition to the variables for both mother and infant. Each infant was followed through his\her clinical file for the first six months of life. Data in the files used to fill the questionnaires to obtain information regarding mother's and infant's sociodemographic profile in addition to the infant history of type of feeding and morbidity during the first six months of life. UNRWA's clinics were used as a place to get the sample for this study because most of refugee camps residents receive their medical care especially MCH free of charge; population utilization of these services is very high due to its location inside refugee camp, and finally patients also visit clinics to obtain medication even if they get medical advice outside these clinics. 3.3.Sample of the study The study sample consisted of clinical records for 690 mother \ infant pairs. The infants were from both genders, reside in one of Nablus 31 refugee camps (Balata, Askar, and Ein Beit el Ma), were born in the year 2007, registered at MCH departments in one of the three clinics included in the study, and met all the study criteria. Mothers of those infants also were included for the study purpose. Files of all infants born between 1\1\2007 to 31\12\2007 were followed for the following 6 months of their birth date. Table (1) below shows the sample distribution according to the place of resident. Table (1): Sample distribution according to the place of resident (n=690) Camp No. of subjects % Askar 284 41.2 Ein Beit el Ma 74 10.7 Balata 332 48.1 From table (1) above we notice that 48.1% of our sample was from Balata camp, while 41.2% and 10.7% were from Askar and Ein Beit el Ma clinics respectively. 3.3.1.Inclusion criteria All inclusion and exclusion criteria were setup to include only healthy term infants, getting their care at the UNRWA clinics as the following:- Infants: i. All infants born in the year 2007. ii. Reside in one of the refugee camps included in the study. 32 iii. Registered at the MCH departments in the clinics. Mothers: All mothers of the study sample infants. 3.3.2. Exclusion criteria For infants i. Infants with low birth weight which is 2500 grams. ii. Premature and preterm infants i.e. infants born before 37 gestational weeks. iii. Those who were born in multiple gestation; twins, triple, etc. iv. Infants with any congenital malformation, chronic disease, or genetic diseases that might affect the study results. v. Infants diagnosed with anemia as been defined by WHO, and utilized by UNRWA s clinics: Mild anemia for Hb < 10 mg\dl. Moderate anemia for Hb 9-9.5 mg\dl. Severe anemia if Hb < 9 mg\dl. vi. Babies diagnosed with immunologic problems. 33 vii. Infants who live outside the camp, because this increase the probability to visit other clinics in Nablus city, or Nablus rural area s rather than the UNRWA's clinics. viii. Infants born after complicated pregnancy such as preeclampsia\ eclampsia and gestational diabetes. ix. Infants with incomplete information in their clinical record files. For mother i. Those with incomplete files. ii. Divorced and widows to ensure that mothers and infants are living in the refugee camp and utilizing its services exclusively. 3.4.Operational definition of the study variables The expected study outcome is focused on the relationship between patterns of infant feeding during the first six months of age and morbidity during the same period of life. At the same time it measures relationship between selected mother s and infant's sociodemographic variables and the choice of different patterns of feeding in the first six months of infant life. Dependent variables: A dependent variable: is what measured in the experiment and what is affected and responds to the independent variable 61. According to the above definition, the followings were considered dependent variables of this study: 34 1. Type of feeding in the first six months of infant life, and according to our study criteria, we defined different methods of feeding for infants in their first six months of life as follow: i. Exclusively breastfed infants (EBF); infants who receive only Breast milk and no water or other fluids (juice, non-human milk) or foods. Only prescribed medicines will be allowed if present. ii. Partially breastfed (PBF); Combination of breast feeding with formula milk, the combination must be started at least from the first week of life, and percentage of breast milk to formula milk will not be taken in consideration in this study because it was not mentioned in the clinical records. iii. Exclusively formula fed infants (EFF); infants receive non-human milk without breast milk. 2. Infant morbidity in the first six months of life, which helps to compare the effects of different types of feeding, especially EBF on infant morbidity in this period of infancy. Morbidity in this study was identified as clinic disease visits which was defined in chapter one. Diseases included were:- upper respiratory tract infection, lower respiratory tract infection, otitis media, asthma, wheezing, gastroenteritis, diarrhea, urinary tract infections, and allergies. 35 Regarding different diseases and diagnosis, the researcher used the entered diagnosis by clinic physicians in the infant files. 3. Frequency of disease visits in the first six months of life in Nablus refugee camps (examples are upper respiratory tract infection, lower respiratory tract infection, otitis media, asthma, wheezing, gastroenteritis, diarrhea, urinary tract infections, and allergies). Independent variables: Independent variable is defined as: The manipulated variable in an experiment or study whose presence or degree determines the change in the dependent variable 62. According to the above definition, the following were considered independent variables. 1. For mother; sociodemographic characteristics regarding each mother in the mother\infant pairs who met selection criteria: i. Mother s age: (15-21 years, 22-28 years, 39-35 years. 36 years and more). ii. Mother s age at marriage: (15-21 years, 22-28 years, 29 years and more). iii. Mother s age when the baby who participated in the study was born: (15-21 years, 22-28 years, 39-35 years. 36 years and more). iv. Parity: (1-2 children, 3-4 children, >4 children) 36 v. Mother s level of education: (not educated, basic, Secondary, high education). vi. Father s (husband) level of education: (not educated, basic, Secondary, high education). This item was added because it was expected that the father also may have a role in promoting the mother decision, especially for those with high level of education. vii. Type of delivery for the infant who participated in the current study: (caesarean, vaginal). 2. For infant; sociodemographic and feeding patterns information for every infant in the mother\infant pair: i. Infant s gender: (male, female). ii. Type of feeding in the first six months of life: (Exclusive Breastfed- EBF, Exclusive Formula Feeding-EFF, or mixture of both \partial breastfeeding-PBF). Notes: During the testing for the specific hypotheses (relationship between the selected mother's and infant's sociodemographic profiles and type of feeding) type of feeding in the first six months of life was considered as a dependent variable to measure the effect of sociodemographic variables on it. While in the first specific hypothesis (relationship between type of feeding and frequency of disease visits) and the main hypothesis (relationship between type of infant feeding and morbidity) it was 37 considered as independent variable to measure how much it affects the morbidity of infant in the first six months of infant s life. 3.5.Instrument of data collection The researcher conducted several visits to the three UNRWA s clinics included in the study. During these visits a comprehensive review of infant\mother files was done to select the suitable ones according to the study criteria. 3.5.1.Questionnaire The researcher developed a three section questionnaire, where important information from each infants\mother file was filled by the researcher herself. a. Section one: contained general information including (Residence, Ration number). b. Section two: contained mother sociodemographic profile including (date of birth, mother s age, mother s age at marriage, mother s age when the infant included in the study was born, parity, mother s and father s levels of education, mother s occupation, mode of delivery for the infant included in the study). c. Section three contained: infant s information including (date of birth, infant s gender, type of feeding in the first six months of life, if the infant has any illness during the first six months of his \ her life, if so, frequency of disease visits, and table for infant s morbidity with 38 different diseases visits in the first six months of life (diseases that was investigated in the study). All mother and infant sociodempographic variables and consequently the questions in the questionnaire were selected according to the study goal and objectives, and according to the literature review. 3.5.2.Reliability and Validity of the tool Reliability is the consistency of the measurement, or the degree to which an instrument measures the same way each time it is used under the same condition with the same subjects 63. Simply reliability is usually estimated by test/retest is that one should get the same score on test 1 as he do on test 2, and internal consistency commonly is by using Cronbach's Alpha 63. Validity refers to whether the questionnaire or survey measures what it intends to measure 64. The study questionnaire developed by the researcher; was based on revision of the literature, and according to study objectives. It was reviewed by the supervisor, and experts, who suggested changes in some items, then all the recommended modifications were done. The researcher her self filled all the questionnaires, and most of the questionnaire s items are social demographic variables and infant morbidity data obtained from mother\infant files. A pilot test was felt to be unnecessary since all data were secondary. 39 3.5.3.Data collection After obtaining a formal approval from UNRWA s director, the researcher herself collected information from clinical files of the three selected UNRWA s clinics between November 2008 to January 2009. The process of data collection went smoothly with very good cooperation of the three clinics staff. 3.6.Potential confounders The economic status might be a confounder, and to adjust for this factor, study sample was chosen exclusively from the three Palestinian refugee camps in Nablus, in which the economic status of families is considered nearly at the same level. However, getting accurate information about income from families at refugee camps is difficult because families will not disclose their actual income for fear of possibility to loose monthly UNRWA s aid. To avoid the effect of confounding factors during analysis, the analysis for the table of the effect of infant feeding on his\her morbidity during the first six months of life was re-examined after applying adjustment for all statistically significant sociodemographic variables. 3.7.Data analysis After collection of data, all information was entered and analyzed using the statistical Microsoft program SPSS version 11 (Statistical Package for Social Sciences). 40 The following statistical formulas were used:- 1. Frequencies and percentages. 2. Crosstab table. 3. Pearson Chi square: is a statistical test used to compare observed data with expected data (data we would expect to obtain according to a specific hypothesis). Our study depends on numbers and frequencies between variables, and examines the relationship between those pairs of variables, therefore; Pearson Chi-square test is thought to be a suitable test. It was used in our study to examine the significance of the relationship between mother and infant demographic variables and type of feeding in the first six months of age. It was also used to study the relationship between type of feeding and morbidity regarding different diseases in the first six months of life. Significance of the relationship is determined when p-value < 0.05. 4. Multiple logistic regression: is used to predict a dependent variable on the basis of continuous and/or categorical independents and to determine the percent of variance in the dependent variable explained by the independents 32. In our study we have some confounding factors like mother s age, infant gender, etc, which must be adjusted to re- examine the relation ship between type of feeding and morbidity in the first six months of infant life again, so the suggested test here is the multiple logistic regression. 41 Accordingly, it was used in our study to re-examine the significance of the relationship between type of feeding and morbidity but after adjusting for mother and infant confounding factors. P-value and adjusted Odds Ratio (adj-OR) determined our result. 3.8.Ethical issues Since this research did not include any meeting with mothers, but collecting secondary data from files by the researcher herself, there is no need to have consents from mothers to conduct the study. Consequently all information was obtained from patient s files after signing an agreement with the UNRWA s administration office. According to this agreement, the researcher abided to all UNRWA's regulations for conducting research and obtaining information including protecting privacy of families records used in this study. Also the researcher obtained the public health department committee's approval from An-najah National university including the ethical consideration. 3.9.Study limitations Although there were no significant obstacles in performing this study, there were some limitations worth mentioning. Limited time of the study, small budget, and the high work load in UNRWA s clinics made it difficult to do face to face or telephone interview. At the same time lack of some other important information in UNRWA s files like family income, mother's smoking, and mother's occupation puts limitation on sociodemographic variables used. 42 Other clinics in Nablus area providing health care to infants (for example ministry of health clinics) are so diverse in term of population, it was difficult to find complete records and follow up for specific infants which made this study unsuitable to be conducted at other clinics than UNRWA s . Therefore, infants from the city and rural areas were not included in the study. Finally, because this study depends on secondary data obtained from clinical files and not face to face meeting, it is expected that the information accuracy may not reach 100%. Summary: This cross-sectional study was conducted in the UNRWA's clinics in Nablus refugee camps; the sample was 690 infant\mother pairs files. A questionnaire was filled by information regarding mother's and infant's socio demographic profile, in addition to information about method of infant feeding and morbidity regarding different types of diseases in the first six month of infant life, data were analyzed using SPSS version 11. Different statistical methods to find frequencies, percentages, and relationships applying Chi-square and multiple logistic regression were used. 43 Chapter Four Results 44 Chapter Four Results This chapter presents in details the results of the study in accordance with its hypotheses. It includes sample distribution according to mother's and infant's sociodemographic profile, sample distribution according to infant s history of morbidity in the first six months. Also it includes sample distribution according to type of infant feeding in the first six months of life, results related to the specific hypotheses, and results related to the main hypothesis before and after adjustment for confounding factors. 4.1. Sample distribution according to mother s and infant s sociodemographic profile Table (2) below shows the sociodemographic profile of the study sample for both mothers and infants. Most of mothers were within the age group (22-28) and (29-35) (39.4% and 33.2%) respectively. For infants, both males and females were equally included in the sample, and majority of them were outcome of vaginal delivery (73.2%). Regarding parity it is noticed that (38.2%) and (36.9%) of mothers in the study sample have (1- 2), and (3-4) children, respectively. Results also indicate that most of the mothers and fathers in our sample had basic and secondary level of education. 45 Table (2): The sociodemographic profile of the study sample for both mothers and infants. (n=690) Variables No. of subjects % Mother's age (15-21) 77 11.2 (22-28) 272 39.4 (29-35) 229 33.2 36 and more 112 16.2 Mother's age at Marriage (15-21) 458 66.4 (22-28) 149 21.6 (29-and more) 83 12.0 Mother's age at baby s birth (15-21) 78 11.4 (22-28) 268 38.8 (29-35) 232 33.6 36 and more 112 16.2 Parity 1-2 264 38.2 3-4 254 36.9 4 172 24.9 Mother's level of education Not educated 42 6.10 Basic 256 37.1 Secondary 270 39.1 High edu cation 122 17.7 Father's level of education(husband) Not educated 44 6.40 Basic 272 39.4 46 Variables No. of subjects % Secondary 261 37.8 High edu cation 113 16.4 Mode of delivery Caesarean 185 26.8 Vaginal 505 73.2 Infant s Gender Male 343 49.7 Female 347 50.3 4.2. Sample distribution according to infant s history of morbidity in the first six months History of morbidity in the first six months of life for all infants included in the study was explored in tables (3), (4). Table (3): Sample distribution according to infant s history of morbidity in the first six months of life (n=690) Variables No. of subjects % Infant have any diseases during the first six months of his \ her life Yes 407 59.0 No 283 41.0 frequency of disease visits None 283 41.0 1 137 19.8 2-4 224 32.5 4 46 6.70 Table (3) shows that out of study sample (690), about 2/3 of infants 407 had documented disease visits during the first six months of life. While 283 (41%) infants did not have any disease during the same period of life. 47 The table also shows that about a third of the study sample (32.5%) had average rate of recurrent visits for disease\s to the clinic (2-4) times. Only (6.7%) of study sample had more than 4 visits for illnesses to the clinic in their first six months of life. Table (4): Sample distribution according to infant s morbidity with different diseases visits in the first six months of life (n = 407) Diseases No. of subjects % Upper respiratory tract infection No 84 20.7 Yes 322 79.3 Lower Respiratory Tract Infection No 370 90.9 Yes 37 9.10 Otitis Media No 352 86.5 Yes 55 13.5 Wheezing No 370 90.9 Yes 37 9.10 Gastroenteritis No 329 80.8 Yes 78 19.2 Diarrhea No 328 80.6 Yes 79 19.6 Urinary tract infection No 401 98.5 Yes 6 1.50 Allergies No 363 89.9 Yes 44 10.1 48 Table (4) shows sample distribution for infants whose files showed a visit for one or more of diseases investigated in the study. It is evident from the table that URTI had the highest frequency (79.3%). On the other hand the lowest was 1.5% of files with diseases for UTI. 4.3. Sample distribution according to type of infant feeding in the first six months of life (n=690) Table (5): Type of feeding in the first six month of life in the study sample (n=690) Type of feeding No. of subjects % Exclusive breastfeeding 481 69.7 Partial breastfeeding 110 15.9 Exclusive formula feeding 99 14.3 Total 690 100% Table (5) shows that in the study sample, most of mothers (69.7%) provided exclusive breastfeeding to the infants in their first six months of life. Partial breastfeeding is less frequently used (15.9%), and exclusive formula feeding is only provided to (14.3%) of infants in the study sample. 4.4. Results related to study hypotheses 4.4.1. Results related to the specific hypotheses relationship between the selected mother's and infant's sociodemographic profiles and type of feeding in the first six months of life in Nablus refugee camps A statistical relationship applying Pearson Chi-Square test (P 0.05) was used to test the different hypotheses between the selected mother's and 49 infant's sociodemographic factors and the type of feeding in the first six months of life. The specific hypothesis stated that "there is no significant relationship, at the significance level p 0.05, between the selected mother s and infant's sociodemographic factors according to (mother's age, mother's age at marriage, mother's age at her baby's birth, parity, mother's level of education, father's level of education, mode of delivery, and infant's gender) and type of feeding in the first six months of life in Nablus refugee camps. Table (6) shows that there was statistically significant relationship between (mother's age, mother's age at marriage, mother's age at her baby's birth, parity, mother's level of education, father's level of education, mode of delivery, and infant's gender) and type of feeding in the first six months of life in Nablus refugee camps. Detailed results for each variable are the followings:- 50 Table (6): Relationship between different mother's and infant's sociodemographic variables and type of feeding in the first six months of life (n=690) Feeding in the first six months of life Correlation EBF PBF EFF Variables No. % No. % No. % Chi- Square Value P-Value 56 72.7 11 14.3 10 13.0 213 .378 47 .317 12 4.40 144 62.9 36 15.7 49 21.4 Mother's age 15-21 22-28 29-35 36 and more 68 60.7 16 14.3 28 .025 46.4 0.00001* 362 .079 75 16.4 21 4.60 104 69.8 29 .519 16 10.7 Mother's age at marriage 15-21 22-28 29 and more 15 18.1 6 7.20 62 .774 285.1 0.00001* 57 73.1 11 14.1 10 12.8 210 .478 46 .217 12 4.50 146 62.9 37 15.9 49 21.1 Mother's age at baby s birth 15-21 22-28 29-35 36 and more 68 60.7 16 14.3 28 .025 45.2 0.00001* 166 62.9 45 17.0 53 .120 181 71.3 35 13.8 38 15.0 Parity 1-2 3-4 4 134 .977 30 .417 8 4.70 22.1 0.001* 25 59.5 13 .031 4 9.50 192 .075 34 13.3 30 11.7 199 .773 39 14.4 32 11.9 Mother's level of education Not educated Basic Secondary High education 65 53.3 24 19.7 33 .027 28.8 0.0001* 35 .579 4 9.1 5 11.4 190 69.9 43 15.8 39 14.3 192 73.6 42 16.1 27 10.3 Father's level of education Not educated Basic Secondary High education 64 56.6 21 .618 28 .824 16.7 0.011* 96 51.9 34 .418 55 .729 Type of delivery Caesarean Vaginal 385 .276 76 15.0 44 8.70 49.4 0.0001* 226 65.9 54 15.7 63 .418 Infant s gender Male Female 255 .573 56 .116 36 10.4 9.2 0.010* * Statically significant at ( = 0.05) 51 A. Mother's age: Regarding mother's age we found that (78.3%) of the mothers at the age (22-28) years breastfed their infant exclusively in the first six months of age, and (17.3%) tends to partially breastfed their infants at the same interval of mother's age, while we found that for the age group 36 years and more the high percentage (25.0%) is for formula feeding (P-value is < 0.05). This means there is a significant correlation between mother s age and type of feeding in the first six months of life. So we reject the hypothesis and say that: There exists a significant relationship, at the level of p 0.05, between mother's age and type of feeding in the first six months of life. B. Mother's age at marriage: For the mother's age at marriage we found that (79.0%) of mothers in the age (15-21) years exclusively breastfed their infants, (19.5%) of the age group (22-28) years partially breastfed their infants, and (74.7%) of the age group 29 years and more were fed their infants by formulas (P-value is < 0.05). This means there is a significant correlation between mother s age at marriage and type of feeding in the first six months of life. So we reject the hypothesis and say that: There exists a significant relationship, at the level of p 0.05, between mother s age at marriage and type of feeding in the first six months of life. 52 C. Mother's age at baby s birth: For this variable, results showed that the high percentage is for the age interval (22-28) years for EBF (78.4%). However it is noticed that age group 36 years tends to have the highest percentage (25%) for EFF infants (P-value is < 0.05). This means there is a significant correlation between mother s age at baby s birth and type of feeding in the first six months of life. So we reject the hypothesis and say that: There exists a significant relationship, at the level of p 0.05, between mother s age at baby s birth and type of feeding in the first six months of life. D. Parity: Regarding this factor the highest percentage (77.9%) is for mothers with parity > 4, compared with mothers with low parity (1-2) children who tended to have highest level for and EFF (20.1%) (P-value is < 0.05). This means there is a significant correlation between parity and type of feeding in the first six months of life. So we reject the hypothesis and say that: There exists a significant relationship, at the level of p 0.05, between parity and type of feeding in the first six months of life. E. Mother's level of education: Study findings show that women with basic and secondary education (75.0%, and 73.0%) tend to have high percentage for EBF compared to non-educated ones and women with high level of education. Women with high education had the highest percent in using EFF (27.0%). which means 53 there is a significant correlation between mother's level of education and type of feeding in the first six months of life (P-value is < 0.05). So we reject the hypothesis and say that: There exists a significant relationship, at the level of p 0.05, between mother's level of education and type of feeding in the first six months of life. F. Father's level of education (husband): Study results indicated that infants born to non-educated fathers tend to have highest percent in receiving exclusive breastfeeding (79.5%), compared to other levels of education. On the other hand infants born to fathers with high education had the highest percent of EFF (24.8%) (P- value is < 0.05). which means there is a significant correlation between father's level of education and type of feeding in the first six months of life (P-value is < 0.05).Therefore, we reject the hypothesis and say that: There exists a significant relationship, at the level of p 0.05, between father's level of education and type of feeding in the first six months of life. G. Type of delivery: Investigation for type of delivery in our research indicated that women who delivered vaginally tended to provide EBF more frequently than those delivered by C-section (76.2% vs 51.9%). EFF was much higher in infants born to mothers through C-section compared to vaginal delivery (P-value is < 0.05). This means there is a significant correlation between type of delivery and type of feeding in the first six months of life. So we 54 reject the hypothesis and say that: There exists a significant relationship, at the level of p 0.05, between type of delivery and type of feeding in the first six months of life. H. Infant's gender: Regarding infant gender, female infants were fed by EBF in higher percent (73.5%) compared to males (65.9%). Higher percent of male infants (18.4%) were given EFF compared to females (P-value is < 0.05). This means there is a significant correlation between infant's gender and type of feeding in the first six months of life. So we reject the hypothesis and say that: There exists a significant relationship, at the level of p 0.05, between infant's gender and type of feeding in the first six months of life. 4.4.2. Results related to the hypotheses of morbidity 4.4.2.1. Results related to the first specific hypothesis relationship between type of feeding and frequency of disease visits in the first six months of life in Nablus refugee camps In order to test the main hypothesis," There exists no significant relationship, at the level of p 0.05, between type of feeding and frequency of disease visits in the first six months of life in Nablus refugee camps (examples are upper respiratory tract infection, lower respiratory tract infection, otitis media, asthma, wheezing, gastroenteritis, diarrhea, urinary tract infections, and allergies) , Pearson Chi-Square test was applied. 55 Tables (7) below shows the frequencies and percentage of the two variables (frequency of disease visits and type of feeding in the first six months of life). Table (7) Relationship between type of feeding in the first six months of life and infant s frequency of disease visits in the same period of age (n=690) Feeding in the first six months of life Correlation EBF PBF EFF Frequency of disease visits No. % No. % No. % Chi-Square Value P-Value 251 .788 16 5.70 16 5.70 111 81.0 19 13.9 7 5.10 112 50.0 56 25.0 56 25.0 None 1 2-4 4 7 15.2 19 41.3 20 43.5 168.9 0.00001* * Statically significant at ( = 0.05) Table (7) shows that (88.7 %) of infants with no disease visits to the clinic were fed by EBF. At the same time those who had high frequent disease visits (> 4) were mainly fed by either PBF (41.3%) or EFF (43.5%). The difference was statistically significant (P 0.05). So we reject the hypothesis and say that there is a significant relationship, at the level of p 0.05, between type of feeding and frequency of disease visits in the first six months of life in Nablus refugee camps. 4.4.2.2. Results related to the main hypothesis relationship between type of infant feeding and morbidity in the first six months of infant s life in Nablus refugee camps In order to test the main hypothesis," There is no significant relationship, at the significance level p 0.05, between type of infant 56 feeding and morbidity in the first six months of infant s life in Nablus refugee camps . Pearson Chi-Square test was applied. Table (8) below shows the relationship between type of infant feeding in the first six months of infant s life and morbidity regarding different diseases in the same age. Table (8): Relationship between type of infant feeding in the first six months of life and morbidity according to different diseases in the same age (n=407) Feeding in the first six months of life Correlation EBF PBF EFF Diseases No. % No. % No. % Chi-Square Value P-Value 47 56.0 16 19.0 21 25.0 Upper respiratory tract infection No Yes 183 56.8 77 23.9 62 19.3 1.8 0.416 221 .759 86 .223 63 .017 Lower respiratory tract infection No Yes 9 24.3 8 21.6 20 54.1 25.6 0.00001* 229 .165 71 .220 52 .817 Otitis Media No Yes 1 1.80 23 41.8 31 56.4 95.2 0.00001* 220 .559 83 .422 67 .118 Wheezing No Yes 10 27.0 11 29.7 16 43.2 16.5 0.00001* 213 .764 58 .617 58 .617 Gastroenteritis No Yes 17 21.8 36 46.2 25 32.1 49.8 0.00001* 206 .862 74 .622 48 .614 Diarrhea No Yes 24 30.4 20 25.3 35 44.3 36.4 0.00001* 217 .895 80 .022 66 .218 Allergies No Yes 13 29.5 14 31.8 17 38.6 15.6 0.00001* *Statically significant at ( = 0.05) Table (8) shows a statistically significant relationship for development of most types of diseases with type of feeding in the first six months of life as follows. 57 A. Upper respiratory tract infection: Regarding this disease we found that P-value was >0.05, which means there is no significant correlation between the type of feeding in the first six months of life and possibility to develop URTI during the same period of age. B. Lower respiratory tract infection: According to findings in table (8), we notice that highest percent for those who did not develop LRTI was for infants fed by EBF (59.7%). On the other hand the highest percentage (54.1%) for those who were diagnosed with LRTI were EFF (P < 0.05). This means that there is a significant correlation between type of feeding and developing LRTI in infants in the first six months of life. C. Otitis media: Numbers show that for infants who did not have OM in the first six months of life EBF was the highest percentage (65.1%). Only (1.8%) of those who developed OM had EBF (P-value is < 0.05). This means that there is a significant correlation between type of feeding and developing otitis media in the first six months of life. D. Wheezing: Regarding wheezing the results found that among infants who had wheezing episode, EFF was the most frequent method of feeding (43.2%). 58 Infants with EBF were the most frequent (59.5%) among those who never wheezed in the first six months of their lives (P < 0.05). Therefore, we can conclude there is a significant correlation between type of feeding and wheezing in the first six months of life. E. Gastroenteritis: For this disease, the study results showed that (64.7%) of infants who did not develop gastroenteritis had EBF, in comparison to (17.6%) for both PBF and EFF (P< 0.05). This means there is a significant correlation between type of feeding and gastroenteritis in the first six months of life. F. Diarrhea: Numbers show that infants who were fed by EFF had the highest percentage in developing diarrhea (44.3%). Infants who did not develop diarrhea were mainly from the EBF group (62.8%) (P-value is < 0.05). This means there is a significant correlation between type of feeding and diarrhea in the first six months of life. G. Allergies: We notice that infants who were breastfed exclusively were the highest to not develop allergies in the first six months of life (59.8%), compared to PBF and EFF (22.0%) and (18.0%) respectively (P-value is < 0.05). This means there is a significant correlation between type of feeding and developing allergies in the first six months of life. 59 In general, the statistical relationship between type of feeding and all diseases in table (8) was significant at P<0.05, except for URTI. Therefore, we reject the hypothesis and say that: There exists a significant relationship, at the level of p 0.05, between type of feeding and morbidity regarding (LRTI, OM, wheezing, gastroenteritis, diarrhea, and allergies) in the first six months of infant s life. Regarding UTI, there were only six reported cases among the study sample (a very small number to apply any statistical association). 4.4.3. Relationship between type of feeding and infant morbidity after adjustment for mother and infant confounding factors After adjustment for mother and infant confounding factors, analysis for morbidity was re-examined, by using multiple logistic regression method. Adjusted Odds Ratio (Adj-OR), 95% confidence intervals, and P- value for significance levels for diseases were investigated. Table (9) below shows the result of analysis, EBF was treated as the referent group. 60 Table (9) Adjusted Odds ratio, Confidence Intervals and Significance levels for diseases affected by type of feeding (n=407) Type of feeding EBF PBF EFF P- value Adj-OR, CI P- value Adj-OR, CI Disease Referent 0.246 1.467 (0.768, 2.801) 0.004* 3.724 (1.528, 9.074) Upper respiratory tract infection Referent 0.421 1.558 (0.530, 4.585) 0.005* 5.110 (1.621, 16.106) Lower respiratory tract infection Referent 0.066 2.506 (0.940, 6.667) 0.006* 4.950 (1.572, 15.625) Wheezing Referent 0.001* 5.525 (2.724, 11.364) 0.050* 2.584 (0.998, 6.711) Gastroenteritis Referent 0.935 1.032 (0.484, 2.202) 0.115 2.033 (0.841, 4.926) Diarrhea Referent 0.019* 2.793 (1.181, 6.624) 0.003* 4.926 (1.704, 14.286) Allergies Adj-OR: adjusted Odds Ratio. CI: Confidence Intervals. * P 0.05, significant From table (9), we notice that after adjusting for mothers' and infants' demographic variables, there is a significant increment in the adjusted odds ratio of EFF and PBF groups for allergies and gastroenteritis. While for URTI, LRTI, and wheezing the increment in adjusted odds ratio reach the significant level only for EFF group. Regarding diarrhea the increased adjusted OR did not reach statistical significance for both EFF and PBF. 61 We omitted OM from analysis after adjustment for confounding factors due to presence of a very small cell in the statistical analysis, which makes relation if present theoretical not practical. Summary The study results show that majority of infants in Nablus refugee camps are fed by EBF ( 70%) in their first six months of life. There exists a significant relationship, at the level of p 0.05, between type of feeding in the first six months of life and mother's and infant's demographic profiles (mother's age, mother's age at marriage, mother's age at her baby's birth, parity, mother's level of education, father's level of education, mode of delivery, and infant's gender). There is also a significant relationship, at the level of p 0.05, between type of feeding and morbidity regarding (LRTI, OM, wheezing, gastroenteritis, diarrhea, and allergies.) in the first six months of infant s life. Even after adjustment for confounding factors this relationship is still significant for (LRTI, wheezing, gastroenteritis, and allergies), in addition to URTI. 62 Chapter Five Discussion 63 Chapter Five Discussion In this chapter, the study result will be discussed in terms of sample distribution, sociodemographic profile of infants and mothers, and the results of tested hypotheses. The study results will be compared by similar studies done globally and regionally. 5.1. Discussion related to sample distribution according to mother s and infant s sociodemographic profile The study sample showed a good distribution of mother s age where it ranged from being very young mothers to older mothers, with the highest percentage was for (22-28) age group (39.4%). Mother s age at marriage shows that early marriage is still a pattern of Palestinian societies in refugee camps. Age of marriage at (15-21) in the sample was (66.4%). It is clear that the trend of early marriage increased in the year 2007, when one noticed in the results of PCBS survey for the year (2006) that the median age at first marriage of ever-married women between the ages (20-54 years) in Palestine refugee camps was for the age 19 years 55. Mother s age at baby s birth showed that the highest percentages were for age groups (22-28) years (38%), then (29-35) years (33.6%). The result agrees with PCBS results of survey (2006); fertility rate per 1000 women by age in Palestine refugee camps, showed that the highest was among the age group (25-29) years (272.3), followed by (20-24)years (229.3) 55. 64 Regarding parity, low parity (1-2) and intermediate (3-4) combined formed around 75% of our sample, leaving high parity group (>4) to be around (25%). According to WHO agenda (2009), parity has stabilized in the last five years, the mean parity among Palestinian refugee women accessing UNRWA services was found to be 4.5 in the West Bank 56. Mother s level of education shows that only (6%) of mothers did not get education and that (17.7%) of mothers received high education. The result goes with PCBS survey (2006), the highest percentages of female (age 10 and above) by educational level in refugee camps were for elementary and preparatory combined (49.8%), and secondary level of education (19.8%) 55. Regarding father s level of education the pattern in this study is similar to mothers level of education; even mothers have slightly higher level of education reflecting that Palestinian society encourages education for both males and females. This results are similar to that of the PCBS survey (2006); the highest percentages distribution of males (age 10 years) by educational level in Palestine refuge camps elementary (22%), then preparatory (30.2%), and finally secondary (17.1%) 55. Regarding type of delivery, about a quarter of study sample had their babies by C-section. This percentage is some what higher than findings in PCBS survey (2006) which pointed that rate of C-section in Nablus was 18.3% 55. However, the annual report of UNRWA (2007); found that the 65 C-section rate among women in West Bank was (27%) 65, a rate close to our findings. For infant gender, males and females were included in equal percentage (49.7% males, 50.3% females) in order to decrease gender bias. This result is highly consistent with PCBS survey (2006), that from population pyramid for the Palestinian Territory we noticed that male and female ratios were (50.7%) and (49.3%) respectively 55. More males are born than females, but males have higher mortality rates than females 55. 5.2. Discussion related to sample distribution according to infant s history of morbidity in the first six months of life Table (3) shows that only (41%) of study sample had no visits for diseases to the clinic. Despite that only (6.7%) had high frequent visits to the clinic (>4). This is usual in the first years of infants life. According to the UNRWA s annual report (2007); attendance for preventive care at UNRWA primary health care facilities (medical examination, immunization, and identification of children with special needs ) during the first year of life was estimated at (90%) of all infants registered agency- wide with the highest percentage (100%) among Palestinian refugees in Lebanon and Gaza fields 65. While the attendance rate agency-wide were (75% and 49%) during the second and third years of infant s life respectively 65. Table (4) shows that URTI was the most frequent for disease visits (79.3%). For healthy infants; this is the mean characteristic of the study 66 sample, URTI is the usual frequent acute illness in infant s life. According to the UNRWA s annual report (2007), respiratory infections is the third main cause of mortality among children aged (1-3) years (14.7%), after congenital malformation and accidents during the year (2006) 65. The least frequent cause for disease visits was UTI and again this is expected for healthy term babies. 5.3. Discussion related to sample distribution according to type of infant feeding in the first six months of life Table (5) shows that around (70%) of the study sample were fed by EBF, and only (14%) were provided EFF. Study results show that Palestinian women in refugee camps tend to provide breastfeeding in a very good percent to their infants. According to the researcher observation, UNRWA s clinics in Nablus refugee camps ( including Balata, Askar, and Ein Beit el Ma) has important and extensive efforts in promoting breastfeeding through the information presented to mothers by nurses in MCH departments, and\or through booklets and pamphlets. This may interpret the large difference in patterns of feeding percentages especially EBF that noticed between this study and PCBS survey (2006); which found that exclusive breastfeeding in the first six months of life in Palestine refugee camps was 24.8% 55. Another explanation for this difference is the poor economic conditions among Palestine refugee camps which may prevent them from getting infant formulas. 67 5.4. Discussion related to study hypotheses 5.4.1. Discussion related to the specific hypotheses relationship between the selected mother's and infant's sociodemographic profiles and type of feeding in the first six months of life in Nablus refugee camps A. Mother's age: This research found that in a society like the Palestinian refugee camps there is a statistically significant association between type of feeding in the first six months of life and mother s age. As noticed in table (6), women aged 22-28 years old were the highest to breastfeed either exclusively (78.3%) or partially (17.3%), while the group aged 36 years old tends to formula feed their infants. These results are in contrary to the results of Irbid study (2003); a semi-urban community in Jordan which found that type of feeding in the first six months of age was independent of mother s age 52, but this study result is similar to that conducted by Sachdev and Mehrptra in New-Delhi; urban city in India (1993-1994), which found that younger mothers were positively related to exclusive breastfeeding 66. On the other hand Ever-Hadani et al study on Jewish women in Jerusalem (1974-1976) found that maternal age < 24 or >40 years old were positively associated with the decision to breastfeed 53. B. Mother's age at marriage: The study results point to the presence of significant relationship between type of feeding in the first six months of infant life and mother s 68 age at marriage (table 6); the younger the mother the higher the tendency to exclusively breastfeed. This result is different from Ever-Hadani et al on Jewish women from Jerusalem (1974-1976), which found that age at marriage did not significantly influence the decision to breastfeed 53. C. Mother's age at baby s birth: In this study (table 6), result point that mother s age at baby s birth correlates significantly with mother s choice about infant type of feeding in the first six months of infant life; mothers aged 22-28 years old were the highest to breastfeed either exclusively or partially, while the group aged 36 years old tend to formula feed their infants. Unfortunately, no previous study was found related to such association. Suggested explanation for the previous three items (mother s age, mother s age at marriage, mother age at baby s birth) may be that marriage and delivery at relatively young age puts high responsibility on mothers to increase their knowledge about maternal and child health in general, and the most suitable way of feeding method for her baby in particular. Therefore, they respond positively to doctors and nurses advice about their infant health more than older mothers. D. Parity: Regarding parity, the result found a statistically significant relation with infant feeding pattern in the fist six months of life (table 6); the higher the parity ( >4 children) the higher the tendency to breastfeed exclusively; 69 and the lower the parity (1-2), the higher the tendency to feed by using formula-milk exclusively. This can be explained by the effect of economic factors to save expenditures on formulas. And may be that practicing breastfeeding is considered as contraceptive method especially for those with high number of children. According to PCBS survey during the year (2004), the percentage of refugee camp women aged (15-49) years who practice breastfeeding as a contraceptive method was 3.7% 29. Another explanation for tendency of mother s of high parity to breastfeed is that high number of children leads to increased experience for mother in breastfeeding. The study findings are consistent with Khassawneh et al study (2003) in Jordan which found that mothers with lower number of children 3 were less likely to exclusively breastfed, and Berger-Achituv study et al in Tel Aviv district which found that grand multiparas ( 5 children) had a significantly higher rate of breast-feeding than women with one to four children 52, 67. While Ever- Hadani et al study in Jerusalem (1974-1976) found that mothers with primipara and grandmultipara >4 breastfeed their infants for longer ( 3 months) 53. E. Mother's level of education: In this study, the results revealed that type of infant s feeding in the