Genetic characterization of some Palestinian figs (Ficus carica L.)
Date
2012-03-25
Authors
Rezq Basheer-Salimia
Murad Awad
Ayed Salama
Saleh Ssekh
Jamil Harb
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
<p>The genetic diversity of 13 local Palestinian fig (Ficus carica L.) genotypes was investigated using RAPD markers. Among the 30 tested primers, 28 revealed various banding patterns and 2 generated no polymorphic bands. In addition, 13 primers (46.4%) produced good amplification products with high intensity and pattern stability. A total of 84 DNA fragments (loci), separated by electrophoresis on agarose gel were detected, ranging in size from 190 to 1300 bp. Of these fragments, 65 (77.4%) were polymorphic and 19 (22.6%) were monomorphic. A minimum of three and a maximum of seven DNA fragments were obtained using (OPH-02 and OPT-10) as well as (Z-08 and OPY-07) primers respectively. The maximum percentage of polymorphic markers was 100.0 (Z-5, Z-12, and OPT- 10) and the minimum was 60.0 (OPH-02). Primers OPY-07 and OPH-19 revealed high collective resolving power (Rp) values with 3.020 and 4.120 respectively and therefore, they were the most useful RAPD primers to assess the genetic diversity in the Palestinian figs. Genetic distance matrix showed an average distance range from 0.155 to 0.557 with a mean of 0.35. Thus, the cultivars tested in this study were characterized by large divergence at the DNA level. To our knowledge, this is the first report using RAPD marker to assess genetic diversity of Palestinian figs.</p>
<p>The genetic diversity of 13 local Palestinian fig (Ficus carica L.) genotypes was investigated using RAPD markers. Among the 30 tested primers, 28 revealed various banding patterns and 2 generated no polymorphic bands. In addition, 13 primers (46.4%) produced good amplification products with high intensity and pattern stability. A total of 84 DNA fragments (loci), separated by electrophoresis on agarose gel were detected, ranging in size from 190 to 1300 bp. Of these fragments, 65 (77.4%) were polymorphic and 19 (22.6%) were monomorphic. A minimum of three and a maximum of seven DNA fragments were obtained using (OPH-02 and OPT-10) as well as (Z-08 and OPY-07) primers respectively. The maximum percentage of polymorphic markers was 100.0 (Z-5, Z-12, and OPT- 10) and the minimum was 60.0 (OPH-02). Primers OPY-07 and OPH-19 revealed high collective resolving power (Rp) values with 3.020 and 4.120 respectively and therefore, they were the most useful RAPD primers to assess the genetic diversity in the Palestinian figs. Genetic distance matrix showed an average distance range from 0.155 to 0.557 with a mean of 0.35. Thus, the cultivars tested in this study were characterized by large divergence at the DNA level. To our knowledge, this is the first report using RAPD marker to assess genetic diversity of Palestinian figs.</p>
<p>The genetic diversity of 13 local Palestinian fig (Ficus carica L.) genotypes was investigated using RAPD markers. Among the 30 tested primers, 28 revealed various banding patterns and 2 generated no polymorphic bands. In addition, 13 primers (46.4%) produced good amplification products with high intensity and pattern stability. A total of 84 DNA fragments (loci), separated by electrophoresis on agarose gel were detected, ranging in size from 190 to 1300 bp. Of these fragments, 65 (77.4%) were polymorphic and 19 (22.6%) were monomorphic. A minimum of three and a maximum of seven DNA fragments were obtained using (OPH-02 and OPT-10) as well as (Z-08 and OPY-07) primers respectively. The maximum percentage of polymorphic markers was 100.0 (Z-5, Z-12, and OPT- 10) and the minimum was 60.0 (OPH-02). Primers OPY-07 and OPH-19 revealed high collective resolving power (Rp) values with 3.020 and 4.120 respectively and therefore, they were the most useful RAPD primers to assess the genetic diversity in the Palestinian figs. Genetic distance matrix showed an average distance range from 0.155 to 0.557 with a mean of 0.35. Thus, the cultivars tested in this study were characterized by large divergence at the DNA level. To our knowledge, this is the first report using RAPD marker to assess genetic diversity of Palestinian figs.</p>